Geneva School of Health Sciences, HES-SO University of Applied Sciences and Arts Western Switzerland, Avenue de Champel 47, 1206 Geneva, Switzerland.
Laboratory of Functional and Metabolic Imaging, Institute of Physics, École Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015 Lausanne, Switzerland.
ACS Chem Neurosci. 2023 Sep 6;14(17):3013-3018. doi: 10.1021/acschemneuro.3c00137. Epub 2023 Aug 21.
Hyperpolarization of C by dissolution dynamic nuclear polarization (dDNP) boosts the sensitivity of magnetic resonance spectroscopy (MRS), making possible the monitoring and in real time of the biochemical reactions of exogenously infused C-labeled metabolic tracers. The preparation of a hyperpolarized substrate requires the use of free radicals as polarizing agents. Although added at very low doses, these radicals are not biologically inert. Here, we demonstrate that the presence of the nitroxyl radical TEMPOL influences significantly the cerebral metabolic readouts of a hyperpolarized [1-C] lactate bolus injection in a mouse model of ischemic stroke with reperfusion. Thus, the choice of the polarizing agent in the design of dDNP hyperpolarized MRS experiments is of great importance and should be taken into account to prevent or to consider significant effects that could act as confounding factors.
通过溶解动态核极化（dDNP）使 C 去极化，提高磁共振波谱（MRS）的灵敏度，使外源性输注的 C 标记代谢示踪剂的生化反应能够实时监测。超极化底物的制备需要使用自由基作为极化剂。尽管这些自由基的添加剂量非常低，但它们并非对生物完全惰性。在这里，我们证明了氮氧自由基 TEMPOL 的存在会显著影响缺血性中风再灌注小鼠模型中 [1-C] 乳酸盐脉冲注射的脑代谢读出值。因此，在设计 dDNP 超极化 MRS 实验时，极化剂的选择非常重要，应考虑到可能作为混杂因素的潜在影响，以防止或考虑这些影响。
