Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region and Key Laboratory of Microbiology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, 150080, China.
Hebei University of Environmental Engineering, Hebei Key Laboratory of Agroecological Safety, Qinhuangdao, 066102, China.
Arch Microbiol. 2023 Aug 21;205(9):313. doi: 10.1007/s00203-023-03652-3.
Surfactin has many biological activities, such as inhibiting plant diseases, resisting bacteria, fungi, viruses, tumors, mycoplasma, anti-adhesion, etc. It has great application potential in agricultural biological control, clinical medical treatment, environmental treatment and other fields. However, the low yield has been the bottleneck of its popularization and application. It is very important to understand the synthesis route and control strategy of surfactin to improve its yield and purity. In this paper, based on the biosynthetic pathway and regulatory factors of surfactin, its biosynthesis regulation strategy was comprehensively summarized, involving enhancement of endogenous and exogenous precursor supply, modification of the synthesis pathway of lipid chain and peptide chain, improvement of secretion and efflux, and manipulation some global regulatory factors, such as Spo0A, AbrB, ComQXP, phrCSF, etc. to directly or indirectly stimulate surfactin synthesis. And the current production and separation and purification process of surfactin are briefly described. This review also provides a scientific reference for promoting surfactin production and its applications in various fields.
表面活性剂具有许多生物活性，如抑制植物病害、抗细菌、真菌、病毒、肿瘤、支原体、抗黏附等。它在农业生物防治、临床医疗、环境处理等领域具有巨大的应用潜力。然而，低产量一直是其推广和应用的瓶颈。了解表面活性剂的合成途径和调控策略对于提高其产量和纯度非常重要。本文基于表面活性剂的生物合成途径和调控因子，综合总结了其生物合成调控策略，涉及增强内源性和外源性前体供应、修饰脂链和肽链的合成途径、改善分泌和外排、以及操纵一些全局调控因子，如 Spo0A、AbrB、ComQXP、phrCSF 等，直接或间接刺激表面活性剂的合成。并简要描述了表面活性剂的当前生产、分离和纯化工艺。本综述还为促进表面活性剂的生产及其在各个领域的应用提供了科学参考。
