Xia Li, Wen Jianping
Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.
National Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin, People's Republic of China.
Crit Rev Biotechnol. 2023 Dec;43(7):1111-1128. doi: 10.1080/07388551.2022.2095252. Epub 2022 Aug 24.
Surfactin is an excellent biosurfactant with a wide range of application prospects in many industrial fields. However, its low productivity and high cost have largely limited its commercial applications. In this review, the pathways for surfactin synthesis in strains are summarized and discussed. Further, the latest strategies for improving surfactin production, including: medium optimization, genome engineering methods (rational genetic engineering, genome reduction, and genome shuffling), heterologous synthesis, and the use of synthetic biology combined with metabolic engineering approaches to construct high-quality artificial cells for surfactin production using xylose, are described. Finally, the prospects for improving surfactin synthesis are discussed in detail.
表面活性素是一种优秀的生物表面活性剂，在许多工业领域具有广泛的应用前景。然而，其低产量和高成本在很大程度上限制了它的商业应用。在这篇综述中，总结并讨论了菌株中表面活性素的合成途径。此外，还描述了提高表面活性素产量的最新策略，包括：培养基优化、基因组工程方法（理性基因工程、基因组缩减和基因组改组）、异源合成，以及利用合成生物学与代谢工程方法相结合构建用于利用木糖生产表面活性素的高质量人工细胞。最后，详细讨论了提高表面活性素合成的前景。
