阿霉素与碳基纳米结构的缀合用于 HT-29 结肠癌细胞的药物传递。
Conjugation of Doxorubicin and Carbon-based-nanostructures for Drug Delivery against HT-29 Colon Cancer Cells.
机构信息
Department of Pharmacy, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
出版信息
Comb Chem High Throughput Screen. 2024;27(18):2726-2733. doi: 10.2174/1386207326666230821145508.
BACKGROUND
A drug delivery system is the method or process of administering a pharmaceutical compound to achieve a therapeutic effect in humans or animals. Such systems release the drugs at specific amounts in a specific site. The carbon based-nanomaterials have been actively used as drug carriers to treat various cancer.
OBJECTIVE
This study aimed to evaluate the cytotoxic effects of DOX-GO, DOX-OMC and DOXCNT in colon cancer cells (HT29).
METHODS
We reported platforms based on graphene oxide (GO), ordered mesoporous carbon (OMC) and carbon nanotubes (CNT) to conjugate with doxorubicin (DOX). The conjugation of DOX with carbon nanomaterial was investigated by UV-Vis spectroscopy, field emission scanning electron microscope (FE-SEM) and cyclic voltammetry (CV) methods.
RESULTS
We showed that graphene oxide was a highly efficient matrix. Efficient loading of DOX, 89%, 78%, and 73.5% at pH 7.0 was seen onto GO, OMC and CNT, respectively. Upon pH 4. 0 after 15 h, 69%, 61% and 61% of DOX could be released from the DOX-GO, DOX-OMC and DOX-CNT, respectively, which illustrated the significant benefits of the developed approach for carbon nanomaterial applications. cytotoxicity analysis showed greater cytotoxicity of DOX/GO, DOX/OMC and DOX/CNT in comparison with GO, OMC and CNT against HT29 colon cancer cells with cell viability of 22%, 40% and 44% after 48 h for DOX-GO, DOX-OMC and DOX-CNT, respectively.
CONCLUSION
The nanohybrids based on DOX-carbon nanomaterial, because of their unique physical and chemical properties, will remarkably enhance the anti-cancer activity.
背景
药物传递系统是将药物化合物施用于人体或动物以达到治疗效果的方法或过程。这样的系统可以在特定部位以特定量释放药物。基于碳的纳米材料已被积极用作药物载体来治疗各种癌症。
目的
本研究旨在评估载多柔比星的 GO（DOX-GO）、载多柔比星的 OMC（DOX-OMC）和载多柔比星的 CNT（DOXCNT）在结肠癌细胞（HT29）中的细胞毒性作用。
方法
我们报道了基于氧化石墨烯（GO）、有序介孔碳（OMC）和碳纳米管（CNT）的平台，以将阿霉素（DOX）与之结合。通过紫外可见光谱、场发射扫描电子显微镜（FE-SEM）和循环伏安法（CV）方法研究了 DOX 与碳纳米材料的结合。
结果
我们表明氧化石墨烯是一种高效的基质。在 pH 7.0 时，GO、OMC 和 CNT 分别高效地将 89%、78%和 73.5%的 DOX 载入。在 pH 4.0 下 15 小时后，分别有 69%、61%和 61%的 DOX 可以从 DOX-GO、DOX-OMC 和 DOX-CNT 中释放出来，这说明了所开发的方法对于碳纳米材料应用的显著优势。细胞毒性分析表明，与 GO、OMC 和 CNT 相比，DOX/GO、DOX/OMC 和 DOX/CNT 对 HT29 结肠癌细胞的细胞毒性更大，DOX-GO、DOX-OMC 和 DOX-CNT 分别在 48 小时后使细胞活力分别降低到 22%、40%和 44%。
结论
由于基于 DOX-碳纳米材料的纳米杂化物具有独特的物理和化学性质，因此将显著提高抗癌活性。
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