Levine S, Saltzman A
J Immunol Methods. 1986 Sep 27;92(2):227-30. doi: 10.1016/0022-1759(86)90170-5.
Injections into rat lymph nodes are easily performed percutaneously, without the need for a skin incision, if the nodes are enlarged in advance. The requisite enlargement of the popliteal lymph node can be obtained in 2 or 3 weeks by a single injection of tin powder or other particulate material into the foot. This method may be especially useful when multiple intranodal inoculations are contemplated.
如果大鼠的淋巴结预先肿大,经皮向其淋巴结注射很容易进行,无需切开皮肤。通过向足部单次注射锡粉或其他颗粒物质,可在2至3周内使腘淋巴结达到所需的肿大程度。当考虑进行多次淋巴结内接种时,这种方法可能特别有用。