Biological Research Laboratories, Nissan Chemical Corporation, Saitama, Japan.
Materials Research Laboratories, Nissan Chemical Corporation, Chiba, Japan.
Biotechnol Lett. 2023 Oct;45(10):1265-1277. doi: 10.1007/s10529-023-03422-7. Epub 2023 Aug 22.
Gene therapy using viral vectors and antibody-based therapies continue to expand within the pharmaceutical market. We evaluated whether Cellhesion VP, a chitin-based material, can be used as 3D culture platform for cell lines used for the production of antibodies and viral vectors.
The results of Cell Counting Kit-8 assay and LDH assay revealed that Cellhesion VP had no adverse effect to Human Embryonic Kidney (HEK) 293, A549 and Chinese hamster ovary (CHO) DG44-Interferon-β (IFN) cells. Cell growth analyses showed that Cellhesion VP supported the 3D culture of HEK293, A549 and CHO DG44- IFN-β cells with a spherical morphology. Importantly, subculture of these cell lines on Cellhesion VP was easily performed without trypsinization because cells readily transferred to newly added scaffold. Our data also suggest that CHO DG44-IFNβ, cultured on Cellhesion VP secreted IFNβ stably and continuously during the culture period.
Cellhesion VP provides a simple and streamlined expansion culture system for the production of biopharmaceuticals.
利用病毒载体和基于抗体的疗法进行基因治疗在制药市场中不断扩展。我们评估了壳聚糖基材料 Cellhesion VP 是否可用作用于生产抗体和病毒载体的细胞系的 3D 培养平台。
Cell Counting Kit-8 检测和 LDH 检测结果表明 Cellhesion VP 对人胚肾（HEK）293、A549 和中国仓鼠卵巢（CHO）DG44-干扰素-β（IFN）细胞没有不良影响。细胞生长分析表明，Cellhesion VP 支持 HEK293、A549 和 CHO DG44-IFN-β细胞的 3D 培养，形成球形形态。重要的是，由于细胞容易转移到新添加的支架上，因此可以轻松地在 Cellhesion VP 上对这些细胞系进行传代培养，而无需使用胰蛋白酶。我们的数据还表明，CHO DG44-IFNβ在 Cellhesion VP 上培养时，在培养期间稳定且持续地分泌 IFNβ。
Cellhesion VP 为生物制药的生产提供了一种简单、精简的扩展培养系统。
