Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China.
Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, Hangzhou, Zhejiang, 310014, China.
Adv Healthc Mater. 2024 Jan;13(2):e2301933. doi: 10.1002/adhm.202301933. Epub 2023 Aug 31.
Drug delivery systems have become a research priority in the biomedical field. The incorporation of liposomes to hydrogels further forms more robust multifunctional systems for more effective and sustained topical drug delivery. In this study, carboxymethyl-modified chitosan/hyaluronic acid (CMC/HA, CMH) thermosensitive hydrogel is developed for sustained transdermal delivery of liposomes. Hydrogels are crosslinked by hydrogen bonding, hydrophobic interaction and electrostatic interaction. The gel properties can be regulated by substitution degree (DS), and when DS = 18.20 ± 0.67% (CMH2), the gel temperature is 37.8 °C, allowing rapid gelation at body temperature (315 s). Moreover, CMH2 hydrogel has suitable spreadability (17.7-57.2 cm ), viscosity (2133.4 mPa s) and porous structure, which facilitated its adhesion and application on the skin and liposomes delivery. The hydrogel can retard the liposomes release, and the release rate of ascorbyl glucoside (AA2G) is 33.92-49.35% in 24 h. Hydrogel avoids the rapid clearance of liposomes from the skin and improved the skin retention, achieving the long-term release of bioactive components. Liposome-hydrogel system more efficiently promotes the anti-photoaging effect of AA2G on skin, reducing epidermal thickness, melanin deposition and lipid oxidative damage and increasing collagen density. Therefore, liposome-hydrogel systems are proposed as multifunctional delivery systems for sustained transdermal delivery.
药物传递系统已成为生物医学领域的研究重点。将脂质体结合到水凝胶中进一步形成更坚固的多功能系统，以实现更有效和持续的局部药物传递。在这项研究中，开发了羧甲基化壳聚糖/透明质酸（CMC/HA，CMH）温敏水凝胶，用于脂质体的持续经皮传递。水凝胶通过氢键、疏水相互作用和静电相互作用交联。凝胶性质可以通过取代度（DS）来调节，当 DS = 18.20 ± 0.67%（CMH2）时，凝胶温度为 37.8°C，允许在体温下快速凝胶化（315 s）。此外，CMH2 水凝胶具有合适的铺展性（17.7-57.2 cm）、粘度（2133.4 mPa s）和多孔结构，便于其在皮肤上粘附和应用以及脂质体的传递。水凝胶可以延缓脂质体的释放，在 24 小时内，AA2G 的释放率为 33.92-49.35%。水凝胶避免了脂质体从皮肤中迅速清除，并提高了皮肤保留率，实现了生物活性成分的长期释放。脂质体-水凝胶系统更有效地促进 AA2G 对皮肤的抗光老化作用，减少表皮厚度、黑色素沉积和脂质氧化损伤，增加胶原蛋白密度。因此，脂质体-水凝胶系统被提议作为用于持续经皮传递的多功能传递系统。
