Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China.
Gynecologic Minimally Invasive Surgery Research Center, Tongji University School of Medicine, 1239 Siping Road, Shanghai, 200092, China.
J Ovarian Res. 2023 Aug 22;16(1):169. doi: 10.1186/s13048-023-01225-y.
Ovarian cancer (OC) is one of the most common gynecologic malignant cancers with the current survival rate remaining low. TRPM2 has been reported as a survival predictor in various cancers but not in OC. The aim of this study is to explore the role and its underlying mechanism of TRPM2 in OC.
The transcriptome data and clinical data were obtained from TCGA, GTEx, and GEO (GSE17260). DriverDBv3 and PrognoScan were used to analyze survival correlations. GSEA analysis was performed to uncover the underlying mechanism. The correlations between TRPM2 and immune score, immune cell infiltration were analyzed by TIMER2.0.
TRPM2 was highly expressed in OC and high TRPM2 expression was related to the poor prognosis based on the Kaplan-Meier curves, univariate and multivariate analysis. The enrichment analysis suggested that TRPM2 was involved in immune-related pathways. Positive correlations were also observed between TRPM2 expression and immune score and immune cells covering B cells, T cells, macrophage, neutrophil, and myeloid dendritic cells. We also found that TRPM2 was positively related to immune checkpoints including ICOSLG, CD40, CD86, etc. TRPM2 expression had a positive correlation with M2 macrophage, but not with M1 macrophage. Besides, TRPM2 showed a strong positive correlation with pyroptosis-related genes including NLRP3, NLRC4, NOD2, NOD1, IL1B, GSDMD.
Our study demonstrated that TRPM2 is a poor prognostic prediction factor in ovarian cancer and is correlated to the immune microenvironment and pyroptosis. TRPM2 may act as a new immunotherapy target, which promoted the survival rate of OC patients.
卵巢癌（OC）是最常见的妇科恶性肿瘤之一，目前的生存率仍然较低。TRPM2 已被报道为各种癌症的生存预测因子，但在 OC 中尚未报道。本研究旨在探讨 TRPM2 在 OC 中的作用及其潜在机制。
从 TCGA、GTEx 和 GEO（GSE17260）获得转录组数据和临床数据。使用 DriverDBv3 和 PrognoScan 分析生存相关性。进行 GSEA 分析以揭示潜在机制。通过 TIMER2.0 分析 TRPM2 与免疫评分、免疫细胞浸润的相关性。
TRPM2 在 OC 中高表达，根据 Kaplan-Meier 曲线、单因素和多因素分析，高 TRPM2 表达与预后不良相关。富集分析表明，TRPM2 参与了免疫相关途径。TRPM2 表达与免疫评分和涵盖 B 细胞、T 细胞、巨噬细胞、中性粒细胞和髓样树突状细胞的免疫细胞之间也存在正相关关系。我们还发现，TRPM2 与免疫检查点包括 ICOSLG、CD40、CD86 等呈正相关。TRPM2 表达与 M2 巨噬细胞呈正相关，但与 M1 巨噬细胞无关。此外，TRPM2 与 NLRP3、NLRC4、NOD2、NOD1、IL1B、GSDMD 等细胞焦亡相关基因呈强正相关。
本研究表明，TRPM2 是卵巢癌的预后不良预测因子，与免疫微环境和细胞焦亡有关。TRPM2 可能作为一种新的免疫治疗靶点，提高 OC 患者的生存率。
