Wu Yue, Zhao Yuwen, Islam Khayrul, Zhou Yuyuan, Omidi Saeed, Berdichevsky Yevgeny, Liu Yaling
Department of Bioengineering, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
ArXiv. 2023 Aug 17:arXiv:2308.06219v2.
Construction of in vitro vascular models is of great significance to various biomedical research, such as pharmacokinetics and hemodynamics, thus is an important direction in tissue engineering. In this work, a standing surface acoustic wave field was constructed to spatially arrange suspended endothelial cells into a designated patterning. The cell patterning was maintained after the acoustic field was withdrawn by the solidified hydrogel. Then, interstitial flow was provided to activate vessel tube formation. Thus, a functional vessel-on-a-chip was engineered with specific vessel geometry. Vascular function, including perfusability and vascular barrier function, was characterized by beads loading and dextran diffusion, respectively. A computational atomistic simulation model was proposed to illustrate how solutes cross vascular lipid bilayer. The reported acoustofluidic methodology is capable of facile and reproducible fabrication of functional vessel network with specific geometry. It is promising to facilitate the development of both fundamental research and regenerative therapy.
构建体外血管模型对各种生物医学研究具有重要意义，如药代动力学和血流动力学研究，因此是组织工程中的一个重要方向。在这项工作中，构建了一个驻波表面声波场，用于将悬浮的内皮细胞在空间上排列成指定的图案。撤去声场后，固化的水凝胶维持了细胞图案。然后，提供间质流以激活血管生成。这样，就构建了一个具有特定血管几何形状的功能性芯片上血管。血管功能，包括灌注性和血管屏障功能，分别通过珠子加载和葡聚糖扩散来表征。提出了一个计算原子模拟模型来说明溶质如何穿过血管脂质双层。所报道的声流体方法能够简便且可重复地制造具有特定几何形状的功能性血管网络。它有望促进基础研究和再生治疗的发展。
