Hung C Y, Burow R D, Scherlag B J, Basmadjian G P, Lazzara R
J Nucl Med. 1986 Oct;27(10):1586-92.
The standard method for measuring myocardial blood flow (MBF) with radioactive microspheres requires processing of selected tissue samples usually from the excised heart, and consequent loss of exact relation to myocardial morphology. A computer-based image processing method was developed by using [99mTc]microspheres (mean particle size 20 microns) for quantitative analysis of MBF in 25 dogs. A computer-controlled gamma camera was used to obtain the images of radioactive microsphere distribution in transaxial slices of the ex vivo heart. Any portion of these slice images could be quantitated by using a computer program based on modification of the formula for determining MBF by the standard microsphere method. Regional myocardial perfusion calculated by this technique correlated well with values obtained with reference microspheres (r = 0.96) over a broad range of MBF. The results show that our new method, accurately and with high resolution, delineated zones of differing MBF and confirmed the increase of MBF in surviving myocardium with healing.
用放射性微球测量心肌血流量（MBF）的标准方法需要对通常取自切除心脏的选定组织样本进行处理，从而失去与心肌形态的确切关系。利用[99mTc]微球（平均粒径20微米）开发了一种基于计算机的图像处理方法，用于对25只犬的MBF进行定量分析。使用计算机控制的γ相机获取离体心脏轴向切片中放射性微球分布的图像。这些切片图像的任何部分都可以通过基于标准微球法测定MBF公式修改的计算机程序进行定量。通过该技术计算的局部心肌灌注在很宽的MBF范围内与参考微球获得的值具有良好的相关性（r = 0.96）。结果表明，我们的新方法准确且具有高分辨率，描绘了不同MBF的区域，并证实了存活心肌中随着愈合MBF的增加。
