Huang S C, Schwaiger M, Carson R E, Carson J, Hansen H, Selin C, Hoffman E J, MacDonald N, Schelbert H R, Phelps M E
J Nucl Med. 1985 Jun;26(6):616-25.
An in vivo measurement technique using 15O water and positron CT for quantitation of myocardial blood flow (MBF) was investigated. A closed-chest dog model and NeuroECAT scanner were used in the study. The in vivo technique involves i.v. infusion of 15O water for a duration of 2-3 min. Oxygen-15 water radioactivity in myocardium was imaged with a NeuroECAT scanner for 10 min, starting at the time of tracer infusion. A separate scan following inhalation of 15O CO was obtained to label the blood pool and to help remove the contribution of radioactivity in the blood pool during the 15O water scans. The integrated projection technique was used for calculating MBF. The quantitative microsphere technique for measurement of MBF was performed along with the 15O water study to provide reference values, with which the MBF values by the in vivo technique was compared. Results of 12 experimental runs (in seven animals) show the in vivo technique with 15O water and positron CT can give quantitative flow images of myocardium. The in vivo positron CT measurement was found to correlate well (r = 0.93) with the in vitro values (by microspheres) over the flow range of 40 to 150 ml/min/100 g.
研究了一种使用(^{15}O)水和正电子计算机断层扫描（CT）定量心肌血流量（MBF）的体内测量技术。该研究使用了闭胸犬模型和NeuroECAT扫描仪。体内技术包括静脉输注(^{15}O)水持续2 - 3分钟。从示踪剂输注时开始，用NeuroECAT扫描仪对心肌中的(^{15}O)水放射性进行10分钟成像。在吸入(^{15}O)一氧化碳后进行单独扫描以标记血池，并有助于去除(^{15}O)水扫描期间血池中放射性的贡献。采用积分投影技术计算MBF。在进行(^{15}O)水研究的同时，采用定量微球技术测量MBF以提供参考值，并将体内技术获得的MBF值与之进行比较。12次实验运行（在7只动物中）的结果表明，使用(^{15}O)水和正电子CT的体内技术可以给出心肌的定量血流图像。发现在40至150毫升/分钟/100克的血流范围内，体内正电子CT测量值与体外值（通过微球）具有良好的相关性（r = 0.93）。
