Department of Biology, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.006006.
A Gram-negative, non-motile and rod-shaped strain, BIT-DXN8, was isolated from the gut of plastic-eating insect larvae . The taxonomic position of this new isolate was examined by using a polyphasic approach. A preliminary analysis based on the 16S rRNA gene sequence (1411 bp) indicated that the most similar strain to BIT-DXN8 was DSM 14964 (98.5%), followed by CIP 64.3 (98.2%) and S23 (98.2%). The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of five housekeeping genes (, , , and ) and genome sequences, placed strain BIT-DXN8 in a separate lineage among the genus of the family . The average nucleotide identity and digital DNA-DNA hybridization values of the strain when compared to all other species within the genus were below 96 and 70 %, respectively. The physiological and biochemical tests confirm the affiliation of strain BIT-DXN8 to the present species within the genus , but with some specific phenotypic differences. Therefore, strain BIT-DXN8 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is BIT-DXN8 (=CCTCC AB 2022117=KCTC 92696).
从食塑昆虫幼虫肠道中分离到一株革兰氏阴性、非运动和杆状的菌株 BIT-DXN8。采用多相鉴定方法研究了该新分离株的分类地位。基于 16S rRNA 基因序列（1411bp）的初步分析表明，与 BIT-DXN8 最相似的菌株为 DSM 14964（98.5%），其次为 CIP 64.3（98.2%）和 S23（98.2%）。基于 16S rRNA 基因、5 个看家基因（、、、和 ）的串联序列和基因组序列的系统发育分析结果表明，菌株 BIT-DXN8 属于 属的一个单独分支。与属内所有其他种相比，菌株 BIT-DXN8 的平均核苷酸同一性和数字 DNA-DNA 杂交值分别低于 96%和 70%。生理生化试验证实，菌株 BIT-DXN8 与该属内的现有种密切相关，但存在一些特定的表型差异。因此，菌株 BIT-DXN8 被认为代表一个新种，建议命名为 sp. nov.。其模式菌株为 BIT-DXN8（=CCTCC AB 2022117=KCTC 92696）。
