Unver Tuba, Erenler Ayse Sebnem, Bingul Murat, Boga Mehmet
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Inonu University, Malatya, Turkey.
Department of Medical Biology, Faculty of Medicine, Turgut Ozal University, Malatya, Turkey.
Chem Biodivers. 2023 Oct;20(10):e202300924. doi: 10.1002/cbdv.202300924. Epub 2023 Sep 8.
Chondroitin synthesis was performed using the recombinant Escherichia coli(C2987) strain created by transforming the plasmid pETM6-PACF-vgb, which carries the genes responsible for chondroitin synthesis, kfoA, kfoC, kfoF, and the Vitreoscilla hemoglobin gene (vgb). Then, Microbial chondroitin sulfate (MCS)'s antioxidant, anticholinesterase, and antibacterial activity were compared with commercial chondroitin sulfate (CCS). The antioxidant studies revealed that the MCS and CCS samples could be potential targets for scavenging radicals and cupric ion reduction. MCS demonstrated better antioxidant properties in the ABTS assay with the IC50 value of 0.66 mg than CCS. MCS showed 2.5-fold for DPPH and almost 5-fold for ABTS⋅+ (with a value of 3.85 mg/mL) better activity than the CCS. However, the compounds were not active for cholinesterase enzyme inhibitions. In the antibacterial assay, the Minimum inhibitory concentration (MIC) values of MCS against S. aureus, E. aerogenes, E. coli, P. aeruginosa, and K. pneumoniae (0.12, 0.18, 0.12, 0.18, and 0.18 g/mL, respectively) were found to be greater than that of CCS (0.42, 0.48, 0.36, 0.36, and 0.36 g/mL, respectively). This study demonstrates that MCS is a potent pharmacological agent due to its physicochemical properties, and its usability as a therapeutic-preventive agent will shed light on future studies.
使用通过转化携带负责软骨素合成的基因kfoA、kfoC、kfoF以及透明颤菌血红蛋白基因（vgb）的质粒pETM6 - PACF - vgb构建的重组大肠杆菌（C2987）菌株进行软骨素合成。然后，将微生物硫酸软骨素（MCS）的抗氧化、抗胆碱酯酶和抗菌活性与商业硫酸软骨素（CCS）进行比较。抗氧化研究表明，MCS和CCS样品可能是清除自由基和还原铜离子的潜在靶点。在ABTS测定中，MCS表现出比CCS更好的抗氧化性能，IC50值为0.66 mg。在DPPH测定中，MCS的活性比CCS高2.5倍，在ABTS·+测定中（值为3.85 mg/mL）几乎高5倍。然而，这些化合物对胆碱酯酶没有抑制活性。在抗菌测定中，发现MCS对金黄色葡萄球菌、产气肠杆菌、大肠杆菌、铜绿假单胞菌和肺炎克雷伯菌的最低抑菌浓度（MIC）值（分别为0.12、0.18、0.12、0.18和0.18 g/mL）高于CCS（分别为0.42、0.48、0.36、0.36和0.36 g/mL）。这项研究表明，由于其理化性质，MCS是一种有效的药理剂，其作为治疗预防剂的可用性将为未来的研究提供启示。
