Unver Tuba, Uslu Harun, Gurhan Ismet, Goktas Bunyamin
Department of Pharmaceutical Microbiology, Faculty of Pharmacy Inonu University Malatya Turkey.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy Fırat University Elazığ Turkey.
Food Sci Nutr. 2024 Jun 24;12(9):6578-6594. doi: 10.1002/fsn3.4251. eCollection 2024 Sep.
The tendency toward natural herbal products has increased due to the antibiotic resistance developed by microorganisms and the severe side effects of antibiotics commonly used in infectious diseases worldwide. Although antimicrobial studies have been conducted with several species of the genus, this study is the first in the literature to be performed with L. subsp. aqueous and methanol extracts. In this study, the phenolic content of was determined by LC-MS/MS analysis, the in vitro antimicrobial activity of aqueous and methanol extracts was examined, and this study was supported by in silico analysis. Consequently, methanol and aqueous extracts were observed to have inhibitory effects against all tested microorganisms except . Although the MIC values of aqueous extract and methanol extract against and are the same (22.5 and 11.25 mg/mL, respectively), the inhibitory effect of aqueous extract is generally more potent (MIC value is 11.25 mg/mL for and other bacterial species, and 90 mg/mL for and ) than that of methanol extract. In silico results showed that hydroxybenzaldeyde, vanillin, resveratrol, isoquercitrin, kaempferol-3-glucoside, fisetin, and luteolin were more prone to antifungal activity. Hence, shikimic, gallic, protocatechuic, vanillic, caffeic, -coumaric, -ferulic, sinapic acids, and hesperidin were more prone to antibacterial activity. In vitro and in silico results show that the antibacterial activity of our extracts may be higher than the antifungal activity. This preliminary study indicates the anti-infective potential of extracts and their usability in medicine and pharmacology.
由于微生物产生的抗生素耐药性以及全球传染病中常用抗生素的严重副作用，对天然草药产品的倾向有所增加。尽管已经对该属的几个物种进行了抗菌研究，但本研究是文献中首次对亚种进行水提取物和甲醇提取物的研究。在本研究中，通过液相色谱 - 质谱/质谱分析测定了的酚类含量，检测了水提取物和甲醇提取物的体外抗菌活性，并通过计算机模拟分析支持了本研究。结果发现，甲醇提取物和水提取物对除之外的所有测试微生物均有抑制作用。尽管水提取物和甲醇提取物对和的最低抑菌浓度（MIC）值相同（分别为22.5和11.25mg/mL），但水提取物的抑制作用通常比甲醇提取物更强（对和其他细菌物种的MIC值为11.25mg/mL，对和的MIC值为90mg/mL）。计算机模拟结果表明，对羟基苯甲醛、香草醛、白藜芦醇、异槲皮苷、山奈酚 - 3 - 葡萄糖苷、非瑟酮和木犀草素更易于产生抗真菌活性。因此，莽草酸、没食子酸、原儿茶酸、香草酸、咖啡酸、对香豆酸、阿魏酸、芥子酸和橙皮苷更易于产生抗菌活性。体外和计算机模拟结果表明，我们提取物的抗菌活性可能高于抗真菌活性。这项初步研究表明提取物的抗感染潜力及其在医学和药理学中的可用性。
