Dey Mohan Kumar, Iftesum Maria, Devireddy Ram, Gartia Manas Ranjan
Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Anal Methods. 2023 Sep 14;15(35):4351-4376. doi: 10.1039/d3ay00844d.
Lateral flow assays (LFAs) are a popular method for quick and affordable diagnostic testing because they are easy to use, portable, and user-friendly. However, LFA design has always faced challenges regarding sensitivity, accuracy, and complexity of the operation. By integrating new technologies and reagents, the sensitivity and accuracy of LFAs can be improved while minimizing the complexity and potential for false positives. Surface enhanced Raman spectroscopy (SERS), photoacoustic techniques, fluorescence resonance energy transfer (FRET), and the integration of smartphones and thermal readers can improve LFA accuracy and sensitivity. To ensure reliable and accurate results, careful assay design and validation, appropriate controls, and optimization of assay conditions are necessary. Continued innovation in LFA technology is crucial to improving the reliability and accuracy of rapid diagnostic testing and expanding its applications to various areas, such as food testing, water quality monitoring, and environmental testing.
侧向流动分析（LFA）是一种用于快速且经济实惠的诊断测试的常用方法，因为它们易于使用、便于携带且对用户友好。然而，LFA的设计在灵敏度、准确性和操作复杂性方面一直面临挑战。通过整合新技术和试剂，可以提高LFA的灵敏度和准确性，同时将复杂性和假阳性的可能性降至最低。表面增强拉曼光谱（SERS）、光声技术、荧光共振能量转移（FRET）以及智能手机与热读仪的整合可以提高LFA的准确性和灵敏度。为确保获得可靠且准确的结果，精心的分析设计与验证、适当的对照以及分析条件的优化是必要的。LFA技术的持续创新对于提高快速诊断测试的可靠性和准确性，并将其应用扩展到食品检测、水质监测和环境检测等各个领域至关重要。
