Laboratory of Deep-sea Microbial Cell Biology, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China; International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille/CAS-Sanya, China; CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China; Institution of Deep-sea Life Sciences, IDSSE-BGI, Hainan Deep-sea Technology Laboratory, China.
Aix Marseille Univ, CNRS, LCB, IMM, IM2B, Marseille, France.
Mar Genomics. 2023 Oct;71:101049. doi: 10.1016/j.margen.2023.101049. Epub 2023 Jun 21.
Tepidibacter sp. SWIR-1, a putative new species isolated from deep-sea hydrothermal vent field on the Southwest Indian Ridge (SWIR), is an anaerobic, mesophilic and endospore-forming bacterium belonging to the family Peptostreptococcaceae. In this study, we present the complete genome sequence of strain SWIR-1, consists of a single circular chromosome comprising 4,122,966 nucleotides with 29.25% G + C content and a circular plasmid comprising 38,843 nucleotides with 29.46% G + C content. In total, 3861 protein coding genes, 104 tRNA genes and 46 rRNA genes were obtained. SWIR-1 genome contains numerous genes related to sporulation and germination. Compared with the other three Tepidibacter species, SWIR-1 contained more spore germination receptor proteins. In addition, SWIR-1 contained more genes involved in chemotaxis and two-component systems than other Tepidibacter species. These results indicated that SWIR-1 has developed versatile adaptability to the Southwest Indian Ridge hydrothermal vent environment. The genome of strain SWIR-1 will be helpful for further understanding adaptive strategies used by bacteria dwelling in the deep-sea hydrothermal vent environments of different oceans.
温暖杆菌属 SWIR-1 株，一种假定的新种，从西南印度洋脊（SWIR）深海热液喷口场中分离得到，是一种厌氧、中温、产芽孢的细菌，属于消化链球菌科。在本研究中，我们介绍了菌株 SWIR-1 的全基因组序列，由一个单一的圆形染色体组成，包含 4122966 个核苷酸，GC 含量为 29.25%，一个圆形质粒包含 38843 个核苷酸，GC 含量为 29.46%。总共获得了 3861 个蛋白编码基因、104 个 tRNA 基因和 46 个 rRNA 基因。SWIR-1 基因组包含许多与孢子形成和萌发相关的基因。与其他三个温暖杆菌种相比，SWIR-1 含有更多的孢子萌发受体蛋白。此外，SWIR-1 比其他温暖杆菌种含有更多参与趋化作用和双组分系统的基因。这些结果表明，SWIR-1 已经发展出了对西南印度洋脊热液喷口环境的多功能适应性。菌株 SWIR-1 的基因组将有助于进一步了解生活在不同海洋深海热液喷口环境中的细菌所采用的适应策略。
