School of Oceanography, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
Mar Genomics. 2022 Jun;63:100936. doi: 10.1016/j.margen.2022.100936. Epub 2022 Mar 7.
Southwest Indian Ridge (SWIR) increasingly becomes the hot spot of deep-sea mining and extreme life research. Strategies for future environmental conservation are undoubtedly required which makes investigation of microbial iron metabolisms imperative. Through deep metagenome sequencing, five iron-oxidizing Zetaproteoabacteria metagenome-assembled genomes were recovered from Longqi hydrothermal vent in the SWIR. Phylogenetic analysis revealed that two of the MAGs might represent novel genus in Zetaproteobacteria while other MAGs were related to Mariprofundus. Functional profile suggested that they might be aerobic chemolithoautotrophic species with genes encoding cytochrome c oxidase, iron oxidase cyc2 homologs and carbon fixation CBB pathway. Versatile capabilities of synthesizing diverse amino acids and cofactors were indicated while possession of various metal ion transporters could be vital to heavy metal resistance. Our work has provided more understanding about phylogenetic and functional features of iron-oxidizing Zetaproteobacteria, which might be important to investigate iron biogeochemistry and mineral oxidation in SWIR.
西南印度洋脊（SWIR）正日益成为深海采矿和极端生命研究的热点。未来的环境保护策略无疑是必要的，这使得对微生物铁代谢的调查变得至关重要。通过深海宏基因组测序，从 SWIR 的龙崎热液喷口回收了五个铁氧化 Zetaproteoabacteria 宏基因组组装基因组。系统发育分析表明，其中两个 MAG 可能代表 Zetaproteobacteria 中的新属，而其他 MAG 则与 Mariprofundus 有关。功能谱表明，它们可能是具有细胞色素 c 氧化酶、铁氧化酶 cyc2 同源物和碳固定 CBB 途径编码基因的需氧化能自养物种。表明它们具有合成各种氨基酸和辅因子的多种能力，而各种金属离子转运蛋白的存在对于重金属抗性可能至关重要。我们的工作提供了对铁氧化 Zetaproteobacteria 的系统发育和功能特征的更多了解，这对于研究 SWIR 中的铁生物地球化学和矿物氧化可能很重要。
