Hsieh Chia-Jung, Hu Chia-Jung, Yu Chi-Yang
Department of Chemical Engineering and Biotechnology, Tatung University, Taipei 104327, Taiwan.
Department of Mechanical and Materials Engineering, Tatung University, Taipei 104327, Taiwan.
Biomimetics (Basel). 2023 Aug 14;8(4):365. doi: 10.3390/biomimetics8040365.
The reaction condition for purifying carbonic anhydrase from (SspCA) by direct heating without prior cell lysis was optimized; heating at 70 °C for 5 min resulted in the highest total activity of 23,460 WAU (Wilbur-Anderson unit) from a 50 mL culture. Heat-purified SspCA was examined for its capability to increase the rate of the mineralization of CO; compared with an uncatalyzed control, the onset time of CaCO formation was shortened by up to 71%. Cyanase can be used to degrade toxic cyanate; however, one of the limitations of this biomimetic process is that the reaction needs HCO as a substrate. Heat-purified SspCA was combined with heat-purified cyanase from to alleviate the HCO dependence; in industrial wastewater, the HCO required was reduced by 50% when 0.75 WAU of SspCA was added. Heat-purified SspCA is stable at 4 °C; 88% of the initial activity was retained for up to five weeks. Partially purified SspCA can be obtained with ease and applied to a variety of applications.
对未经预先细胞裂解直接加热从嗜盐栖热栖热袍菌（SspCA）中纯化碳酸酐酶的反应条件进行了优化；在70℃加热5分钟，从50毫升培养物中获得了最高总活性23,460 WAU（威尔伯-安德森单位）。对热纯化的SspCA提高CO矿化速率的能力进行了检测；与未催化的对照相比，CaCO形成的起始时间缩短了多达71%。氰酸酶可用于降解有毒氰酸盐；然而，这种仿生过程的局限性之一是该反应需要HCO作为底物。将热纯化的SspCA与嗜盐栖热栖热袍菌的热纯化氰酸酶结合以减轻对HCO的依赖性；在工业废水中，添加0.75 WAU的SspCA时，所需的HCO减少了50%。热纯化的SspCA在4℃下稳定；长达五周可保留88%的初始活性。部分纯化的SspCA易于获得并可应用于多种用途。
