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代谢：柚皮素类似物的分离、鉴定以及与纳米颗粒干预相关的上调基因

Metabolism: Isolation, Identification of Naringenin Analogues and Genes Elevated Associated with Nanoparticle Intervention.

作者信息

Al-Theyab Nada S, Abuelizz Hatem A, Al-Hamoud Gadah A, Aldossary Ahmad, Liang Mingtao

机构信息

School of Biomedical Science and Pharmacy, University of Newcastle, Callaghan, NSW 2308, Australia.

Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

出版信息

Curr Issues Mol Biol. 2023 Aug 14;45(8):6704-6716. doi: 10.3390/cimb45080424.

DOI:10.3390/cimb45080424
PMID:37623243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10453022/
Abstract

The impact of gold nanoparticles (AuNPs) on the biosynthetic manipulation of metabolism where an existing gene cluster is enhanced to produce and enrich bioactive secondary metabolites has been studied previously. In this research, we aimed to isolate and elucidate the structure of metabolites of compounds 1 and 2 which have been analyzed previously in crude extract. This was achieved through a PREP-ODS C18 column with an HPLC-UV/visible detector. Then, the compounds were subjected to nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS), and Fourier-transform infrared spectroscopy (FT-IR) techniques. Furthermore, bioinformatics and transcriptome analysis were used to examine the gene expression for which the secondary metabolites produced in the presence of AuNPs showed significant enhancement in transcriptomic responses. The metabolites of compounds 1 and 2 were identified as daidzein and genistein, respectively. The real-time polymerase chain reaction (RT-PCR) technique was used to assess the expression of three genes (, , and ) from a panel of selected genes known to be involved in the biosynthesis of the identified secondary metabolites. The expression levels of two genes ( and ) increased in response to AuNP intervention, whereas was unaffected.

摘要

此前已研究了金纳米颗粒（AuNPs）对代谢生物合成操纵的影响，即增强现有基因簇以产生和富集生物活性次生代谢产物。在本研究中，我们旨在分离并阐明化合物1和2的代谢产物结构，这些化合物先前已在粗提物中进行了分析。这是通过配备HPLC-UV/可见光检测器的PREP-ODS C18柱实现的。然后，对这些化合物进行核磁共振（NMR）、电喷雾电离质谱（ESI-MS）和傅里叶变换红外光谱（FT-IR）技术分析。此外，利用生物信息学和转录组分析来检测基因表达，在金纳米颗粒存在下产生的次生代谢产物在转录组反应中显示出显著增强。化合物1和2的代谢产物分别被鉴定为大豆苷元和染料木黄酮。使用实时聚合酶链反应（RT-PCR）技术评估一组已知参与所鉴定次生代谢产物生物合成的选定基因中的三个基因（、和）的表达。两个基因（和）的表达水平因金纳米颗粒干预而升高，而未受影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/711ca0f5fd5a/cimb-45-00424-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/48080486a332/cimb-45-00424-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/fa4ba48da251/cimb-45-00424-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/183e67be1b8e/cimb-45-00424-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/2e142b8469a0/cimb-45-00424-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/abc799e7ee43/cimb-45-00424-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/fc4ef7f26948/cimb-45-00424-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/b50c3b95f470/cimb-45-00424-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/711ca0f5fd5a/cimb-45-00424-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/48080486a332/cimb-45-00424-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/fa4ba48da251/cimb-45-00424-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/183e67be1b8e/cimb-45-00424-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/2e142b8469a0/cimb-45-00424-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/abc799e7ee43/cimb-45-00424-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/fc4ef7f26948/cimb-45-00424-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/b50c3b95f470/cimb-45-00424-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/10453022/711ca0f5fd5a/cimb-45-00424-g008.jpg

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