Al-Theyab Nada, Alrasheed Omar, Abuelizz Hatem A, Liang Mingtao
School of Biomedical Science and Pharmacy, University of Newcastle, Callaghan, New South Wales, Australia.
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Saudi Pharm J. 2023 Jun;31(6):783-794. doi: 10.1016/j.jsps.2023.04.016. Epub 2023 Apr 23.
Insights about the effects of gold nanoparticles (AuNPs) on the biosynthetic manipulation of unknown microbe secondary metabolites could be a promising technique for prospective research on nano-biotechnology.
In this research, we aimed to isolate a fresh, non-domesticated unknown bacterium strain from a common scab of potato crop located in Saudi Arabia and study the metabolic profile.
This was achieved through genomic DNA (gDNA) sequencing using Oxford Nanopore Technology. The genomic data were subjected to several bioinformatics tools, including canu-1.9 software, Prokka, DFAST, Geneious Prime, and AntiSMASH. We exposed the culture of the bacterial isolate with different concentrations of AuNPs and investigated the effects of AuNPs on secondary metabolites biosynthesis using several analytical techniques. Furthermore, Tandem-mass spectrometric (MS/MS) technique was optimized for the characterization of several significant sub-classes.
The genomic draft sequence assembly, alignment, and annotation have verified the bacterial isolate as . This bacterium has secondary metabolites related to different biosynthetic gene clusters. AuNPs intervention showed an increase in the production of compounds with the molecular weights of 254 and 270 Da in a direct-dependent manner with the increase of the AuNPs concentrations.
The increase in the yields of compound 1 and 2 concomitantly with the increase in the concentration of the added AuNPs provide evidences about the effects of nanoparticles on the biosynthesis of the secondary metabolites. It contributes to the discovery of genes involved in different biosynthetic gene clusters (BGCs) and prediction of the structures of the natural products.
关于金纳米颗粒（AuNPs）对未知微生物次级代谢产物生物合成操纵影响的见解，可能是纳米生物技术前瞻性研究的一项有前景的技术。
在本研究中，我们旨在从沙特阿拉伯的马铃薯疮痂病中分离出一种新鲜的、未驯化的未知细菌菌株，并研究其代谢谱。
这是通过使用牛津纳米孔技术进行基因组DNA（gDNA）测序来实现的。基因组数据使用了多种生物信息学工具，包括canu - 1.9软件、Prokka、DFAST、Geneious Prime和AntiSMASH。我们用不同浓度的AuNPs处理细菌分离株的培养物，并使用多种分析技术研究AuNPs对次级代谢产物生物合成的影响。此外，串联质谱（MS/MS）技术针对几个重要的亚类进行了优化表征。
基因组草图序列组装、比对和注释已将该细菌分离株鉴定为 。这种细菌具有与不同生物合成基因簇相关的次级代谢产物。AuNPs干预显示，随着AuNPs浓度的增加，分子量为254和270 Da的化合物产量呈直接依赖性增加。
化合物1和2的产量随着添加的AuNPs浓度增加而增加，这为纳米颗粒对次级代谢产物生物合成的影响提供了证据。它有助于发现参与不同生物合成基因簇（BGCs）的基因，并预测天然产物的结构。
