Shiri Tanisha Jean, Viau Charles, Gu Xue, Xu Lei, Lu Yao, Xia Jianguo
Institute of Parasitology, McGill University, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada.
Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.
Metabolites. 2023 Aug 18;13(8):953. doi: 10.3390/metabo13080953.
Aging is the system-wide loss of homeostasis, eventually leading to death. There is growing evidence that the microbiome not only evolves with its aging host, but also directly affects aging via the modulation of metabolites involved in important cellular functions. The widely used model organism exhibits high selectivity towards its native microbiome members which confer a range of differential phenotypes and possess varying functional capacities. The ability of one such native microbiome species, sp. CHNTR56 MYb120, to improve the lifespan of and to promote the production of Vitamin B6 in the co-colonizing member sp. 12022 MYb131 are some of its beneficial effects on the worm host. We hypothesize that studying its metabolic influence on the different life stages of the worm could provide further insights into mutualistic interactions. The present work applied LC-MS untargeted metabolomics and isotope labeling to study the impact of the native microbiome member sp. CHNTR56 MYb120 on the metabolism of . In addition to the upregulation of biosynthesis and detoxification pathway intermediates, we found that sp. CHNTR56 MYb120 upregulates the glyoxylate shunt in mid-adult worms which is linked to the upregulation of trehalose, an important metabolite for desiccation tolerance in older worms.
衰老指的是体内稳态在全身范围内的丧失，最终导致死亡。越来越多的证据表明，微生物群不仅会随着衰老的宿主一同进化，还会通过调节参与重要细胞功能的代谢物直接影响衰老过程。这种广泛使用的模式生物对其天然微生物群成员表现出高度选择性，这些成员赋予了一系列不同的表型，并具有不同的功能能力。其中一种天然微生物群物种，即CHNTR56 MYb120菌，能够延长线虫的寿命，并促进共同定殖的12022 MYb131菌产生维生素B6，这些都是它对线虫宿主的一些有益作用。我们假设，研究其对线虫不同生命阶段的代谢影响，可能会为共生相互作用提供进一步的见解。本研究应用液相色谱 - 质谱非靶向代谢组学和同位素标记技术，来研究天然微生物群成员CHNTR56 MYb120菌对线虫代谢的影响。除了生物合成和解毒途径中间体的上调外，我们还发现CHNTR56 MYb120菌上调了中年线虫的乙醛酸循环途径，这与海藻糖的上调有关，海藻糖是老年线虫抗干燥的一种重要代谢物。
