Kang Dingrong, Shoaie Saeed, Jacquiod Samuel, Sørensen Søren J, Ledesma-Amaro Rodrigo
Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark.
Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
Microorganisms. 2021 May 12;9(5):1042. doi: 10.3390/microorganisms9051042.
A promising keratin-degrading strain from the genus ( sp. KMC2) was investigated using comparative genomic tools against three publicly available reference genomes to reveal the keratinolytic potential for biosynthesis of valuable secondary metabolites. Genomic features and metabolic potential of four species were compared, showing genomic differences but similar functional categories. Eleven different secondary metabolite gene clusters of interest were mined from the four genomes successfully, including five common ones shared across all genomes. Among the common metabolites, we identified gene clusters involved in biosynthesis of flexirubin-type pigment, microviridin, and siderophore, showing remarkable conservation across the four genomes. Unique secondary metabolite gene clusters were also discovered, for example, ladderane from sp. KMC2. Additionally, this study provides a more comprehensive understanding of the potential metabolic pathways of keratin utilization in sp. KMC2, with the involvement of amino acid metabolism, TCA cycle, glycolysis/gluconeogenesis, propanoate metabolism, and sulfate reduction. This work uncovers the biosynthesis of secondary metabolite gene clusters from four keratinolytic species and shades lights on the keratinolytic potential of sp. KMC2 from a genome-mining perspective, can provide alternatives to valorize keratinous materials into high-value bioactive natural products.
利用比较基因组工具，针对三个公开可用的参考基因组，对一种有前景的来自（属名）属的角蛋白降解菌株（sp. KMC2）进行了研究，以揭示其在生物合成有价值的次生代谢产物方面的角蛋白分解潜力。比较了四个物种的基因组特征和代谢潜力，结果显示基因组存在差异，但功能类别相似。成功地从这四个基因组中挖掘出了11个不同的感兴趣的次生代谢产物基因簇，其中包括所有基因组共有的5个。在这些共同的代谢产物中，我们鉴定出了参与flexirubin型色素、微病毒素和铁载体生物合成的基因簇，这些基因簇在四个基因组中表现出显著的保守性。还发现了独特的次生代谢产物基因簇，例如来自sp. KMC2的梯形烷。此外，本研究更全面地了解了sp. KMC2中角蛋白利用的潜在代谢途径，涉及氨基酸代谢、三羧酸循环、糖酵解/糖异生、丙酸代谢和硫酸盐还原。这项工作揭示了四种角蛋白分解（属名）物种的次生代谢产物基因簇的生物合成，并从基因组挖掘的角度揭示了sp. KMC2的角蛋白分解潜力，可为将角蛋白材料转化为高价值生物活性天然产物提供替代方法。
