Amanatidou Athina I, Mikropoulou Eleni V, Amerikanou Charalampia, Milanovic Maja, Stojanoski Stefan, Bjelan Mladen, Cesarini Lucia, Campolo Jonica, Thanopoulou Anastasia, Banerjee Rajarshi, Kurth Mary Jo, Milic Natasa, Medic-Stojanoska Milica, Trivella Maria Giovanna, Visvikis-Siest Sophie, Gastaldelli Amalia, Halabalaki Maria, Kaliora Andriana C, Dedoussis George V
Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, 17671 Athens, Greece.
Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Metabolites. 2023 Aug 18;13(8):959. doi: 10.3390/metabo13080959.
Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) have been linked to changes in amino acid (AA) levels. The objective of the current study was to examine the relationship between MRI parameters that reflect inflammation and fibrosis and plasma AA concentrations in NAFLD patients. Plasma AA levels of 97 NAFLD patients from the MAST4HEALTH study were quantified with liquid chromatography. Medical, anthropometric and lifestyle characteristics were collected and biochemical parameters, as well as inflammatory and oxidative stress biomarkers, were measured. In total, subjects with a higher MRI-proton density fat fraction (MRI-PDFF) exhibited higher plasma AA levels compared to subjects with lower PDFF. The concentrations of BCAAs (-Value: 0.03), AAAs (-Value: 0.039), L-valine (-Value: 0.029), L-tyrosine (-Value: 0.039) and L-isoleucine (-Value: 0.032) were found to be significantly higher in the higher PDFF group compared to lower group. Plasma AA levels varied according to MRI-PDFF. Significant associations were also demonstrated between AAs and MRI-PDFF and MRI-cT1, showing the potential utility of circulating AAs as diagnostic markers of NAFLD.
非酒精性脂肪性肝病（NAFLD）和非酒精性脂肪性肝炎（NASH）与氨基酸（AA）水平的变化有关。本研究的目的是探讨反映炎症和纤维化的MRI参数与NAFLD患者血浆AA浓度之间的关系。对MAST4HEALTH研究中97例NAFLD患者的血浆AA水平进行液相色谱定量分析。收集患者的医学、人体测量和生活方式特征，并检测生化参数以及炎症和氧化应激生物标志物。总体而言，与质子密度脂肪分数（MRI-PDFF）较低的受试者相比，MRI-PDFF较高的受试者血浆AA水平更高。与较低组相比，较高PDFF组的支链氨基酸（-值：0.03）、芳香族氨基酸（-值：0.039）、L-缬氨酸（-值：0.029）、L-酪氨酸（-值：0.039）和L-异亮氨酸（-值：0.032）浓度显著更高。血浆AA水平随MRI-PDFF而变化。AA与MRI-PDFF和MRI-cT1之间也存在显著关联，表明循环AA作为NAFLD诊断标志物的潜在效用。
