Cetintepe Sultan Pınar, Hazar Merve, Bilinmiş Izem, Aydin Dilsiz Sevtap, Basaran Nursen
Department of Public Health, Faculty of Medicine, Gazi University, 06500, Ankara, Turkiye.
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ağrı İbrahim Çeçen University, Ağrı, 04100, Turkiye.
Environ Res. 2023 Nov 15;237(Pt 1):116970. doi: 10.1016/j.envres.2023.116970. Epub 2023 Aug 23.
The automotive industry is a very wide area from the manufacturing of the pieces of the engine, the body, plastics to the assembly of the car. There is a chemical risk at different stages of production because of the requirement of the use of many corrosive and irritant chemicals such as paints, adhesives, acids, and bases. The aim of the study was to determine the genotoxicity, oxidative stress and immune parameters of automotive paint workers in Ankara, Türkiye. DNA damage of workers mainly responsible from the painting of the automotives were evaluated using the alkaline comet assay and the levels of some oxidative stress and immune biomarkers were also investigated. Increased lymphocyte DNA damage and also higher 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels were observed while decreased glutathione (GSH), glutathione peroxidase (GPx), and glutathione reductase (GR) levels were found in the workers compared to their controls There were no significant differences between the study groups in the levels of interleukin (IL)- 1beta, IL-17, IL-23, Clara cell secretory protein (CC16), tumor necrosis factor-alpha (TNF-alpha), catalase (CAT), and superoxide dismutase (SOD). The results show that occupational exposure to chemicals in automotive industry may cause DNA damage in workers due to oxidative stress.
汽车行业涵盖的领域非常广泛，从发动机部件制造、车身制造、塑料制品到汽车组装。由于在生产的不同阶段需要使用许多腐蚀性和刺激性化学品，如油漆、粘合剂、酸和碱，因此存在化学风险。本研究的目的是确定土耳其安卡拉汽车喷漆工人的遗传毒性、氧化应激和免疫参数。使用碱性彗星试验评估主要负责汽车喷漆工作的工人的DNA损伤，并研究一些氧化应激和免疫生物标志物的水平。与对照组相比，观察到工人的淋巴细胞DNA损伤增加，同时8-羟基-2'-脱氧鸟苷（8-OHdG）和丙二醛（MDA）水平升高，而谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）水平降低。研究组之间白细胞介素（IL）-1β、IL-17、IL-23、克拉拉细胞分泌蛋白（CC16）、肿瘤坏死因子-α（TNF-α）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）水平无显著差异。结果表明，汽车行业的职业性化学物质暴露可能由于氧化应激导致工人DNA损伤。
