Pordel Edris, Ghasemi Trife, Afrasiabi Shima, Benedicenti Stefano, Signore Antonio, Chiniforush Nasim
Department of Pediatric Dentistry, Dental School, Sabzevar University of Medical Sciences, Sabzevar 9613875389, Iran.
Independent Researcher, Mashhad 9613875389, Iran.
Biomedicines. 2023 Aug 10;11(8):2248. doi: 10.3390/biomedicines11082248.
The aim of the present study was to determine the effects of antimicrobial photodynamic therapy (aPDT) using the blue diode laser (BDL) with different output powers and the photosensitizers riboflavin and curcumin on reducing the number of around orthodontic brackets.
A total of 36 orthodontic brackets were contaminated with and randomly assigned to 12 groups as follows: control, riboflavin alone, riboflavin + BDL with an output power of 200, 300, 400, or 500 mW, and curcumin alone, curcumin + BDL with an output power of 200, 300, 400, or 500 mW, and 0.2% chlorhexidine (CHX-positive control). Orthodontic brackets were irradiated with a BDL (wavelength 445 nm) at a power density of 0.4-1.0 W/cm for 30 s. All orthodontic brackets were examined under a stereomicroscope at 10× magnification. Mean colony-forming units (CFUs)/mL were measured before and after treatment. A one-way analysis of variance with Tukey's post hoc test was performed to compare CFU/mL between groups.
CHX and curcumin plus BDL with an output power of 500 mW had the highest reduction in colony numbers ( < 0.001). The curcumin groups were more effective than the riboflavin groups. Riboflavin alone and riboflavin + BDL with an output power of 200 mW showed no significant difference from the control group ( = 0.99 and 0.74, respectively).
Our results suggest that aPDT using curcumin as a photosensitizer plus BDL with an output power of 500 mW and a power density of 1.0 W/cm at a wavelength of 445 nm can effectively reduce colonies of around stainless steel brackets.
本研究旨在确定使用不同输出功率的蓝光二极管激光（BDL）以及光敏剂核黄素和姜黄素的抗菌光动力疗法（aPDT）对减少正畸托槽周围[细菌名称未给出]数量的影响。
总共36个正畸托槽被[细菌名称未给出]污染，并随机分为12组，如下：对照组、单独使用核黄素组、核黄素 + 输出功率为200、300、400或500 mW的BDL组，以及单独使用姜黄素组、姜黄素 + 输出功率为200、300、400或500 mW的BDL组，还有0.2%氯己定（CHX阳性对照组）。使用BDL（波长445 nm）以0.4 - 1.0 W/cm的功率密度照射正畸托槽30秒。所有正畸托槽在10倍放大率的体视显微镜下检查。在治疗前后测量平均菌落形成单位（CFU）/mL。进行单因素方差分析并采用Tukey事后检验来比较各组之间的CFU/mL。
CHX以及输出功率为500 mW的姜黄素加BDL组在[细菌名称未给出]菌落数量减少方面最为显著（P < 0.001）。姜黄素组比核黄素组更有效。单独使用核黄素组以及输出功率为200 mW的核黄素 + BDL组与对照组无显著差异（分别为P = 0.99和0.74）。
我们的结果表明，以姜黄素作为光敏剂加输出功率为500 mW、波长445 nm、功率密度1.0 W/cm的BDL的aPDT可以有效减少不锈钢托槽周围的[细菌名称未给出]菌落。
