Morelato Luka, Budimir Ana, Smojver Igor, Katalinić Ivan, Vuletić Marko, Ajanović Muhamed, Gabrić Dragana
Faculty of Dental Medicine, University of Rijeka, 51000 Rijeka, Croatia.
Department of Clinical and Molecular Microbiology, School of Medicine, University Hospital Centre Zagreb, University of Zagreb, 10000 Zagreb, Croatia.
Bioengineering (Basel). 2022 Jul 12;9(7):308. doi: 10.3390/bioengineering9070308.
Antimicrobial photodynamic therapy (PDT) has been introduced as a potential option for peri-implantitis treatment. The aim of this study is to evaluate the antimicrobial effect of a novel technique involving a combination of 445 nm diode laser light with 0.1% riboflavin solution (used as a photosensitizing dye) as applied on a bacterial-fungal biofilm formed on implants and to compare the performance of this technique with that of the commonly used combination of 660 nm diode laser with 0.1% methylene blue dye.
An in vitro study was conducted on 80 titanium dental implants contaminated with (SA) and (CA) species. The implants were randomly divided into four groups: negative control (NC), without surface treatment; positive control (PC), treated with a 0.2% chlorhexidine (CHX)-based solution; PDT1, 660 nm (EasyTip 320 µm, 200 mW, Q power = 100 mW, 124.34 W/cm, 1240 J/cm) with a 0.1% methylene blue dye; and PDT2, 445 nm (EasyTip 320 µm, 200 mW, Q power = 100 mW, 100 Hz, 124.34 W/cm, 1.24 J/cm) with a 0.1% riboflavin dye.
The PDT1 and PDT2 groups showed greater reduction of SA and CA in comparison to the NC group and no significant differences in comparison to the PC group. No statistically significant differences between the PDT1 and PDT2 groups were observed.
A novel antimicrobial treatment involving a combination of 445 nm diode laser light with riboflavin solution showed efficiency in reducing SA and CA biofilm formation on dental implant surfaces comparable to those of the more commonly used PDT treatment consisting of 660 nm diode laser light with methylene blue dye or 0.2% CHX treatment.
抗菌光动力疗法（PDT）已被引入作为种植体周围炎治疗的一种潜在选择。本研究的目的是评估一种新技术的抗菌效果，该技术将445nm二极管激光与0.1%核黄素溶液（用作光敏染料）结合应用于种植体上形成的细菌 - 真菌生物膜，并将该技术的性能与常用的660nm二极管激光与0.1%亚甲蓝染料的组合进行比较。
对80颗被金黄色葡萄球菌（SA）和白色念珠菌（CA）污染的钛制牙种植体进行体外研究。将种植体随机分为四组：阴性对照组（NC），不进行表面处理；阳性对照组（PC），用0.2%氯己定（CHX）溶液处理；PDT1组，660nm（EasyTip 320µm，200mW，Q功率 = 100mW，124.34W/cm，1240J/cm）结合0.1%亚甲蓝染料；PDT2组，445nm（EasyTip 320µm，200mW，Q功率 = 100mW，100Hz，124.34W/cm，1.24J/cm）结合0.1%核黄素染料。
与NC组相比，PDT1组和PDT2组的SA和CA减少更多，与PC组相比无显著差异。PDT1组和PDT2组之间未观察到统计学上的显著差异。
一种将445nm二极管激光与核黄素溶液结合的新型抗菌治疗方法在减少牙种植体表面SA和CA生物膜形成方面显示出与更常用的由660nm二极管激光与亚甲蓝染料组成的PDT治疗或0.2%CHX治疗相当的效率。
