Bergsma Anna T, Li Hui Ting, Eliveld Jitske, Bulthuis Marian L C, Hoek Annemieke, van Goor Harry, Bourgonje Arno R, Cantineau Astrid E P
Department of Obstetrics and Gynecology, University of Groningen, University Medical Centre Groningen, 9700 RB Groningen, The Netherlands.
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, 9713 GZ Groningen, The Netherlands.
Antioxidants (Basel). 2022 May 25;11(6):1045. doi: 10.3390/antiox11061045.
Infertility problems occur in around 10% of all couples worldwide, with male-factor infertility as the sole contributor in 20-30% of these cases. Oxidative stress (OS) is suggested to be associated with the pathophysiology of male infertility. In spermatozoa, OS can lead to damage to the cell membrane, resulting in disruption of DNA integrity and a decrease in motility. Established biomarkers for OS include free thiols and malondialdehyde (MDA), both representing different components of the reactive species interactome (RSI). This exploratory study aimed to investigate seminal plasma-free thiol and MDA levels in relation to semen parameters as defined by the World Health Organization (WHO) to determine if these markers are adequate to define local OS status. Furthermore, this study investigated if there is a relation between systemic and local OS status by comparing seminal concentrations of free thiol (R-SH, sulfhydryl groups, representing the extracellular redox status) and MDA (lipid peroxidation product) levels to those measured in serum. Free thiol and MDA measurements in both serum and semen plasma were performed in 50 males (18-55 y) of couples seeking fertility treatment. A significant positive correlation was found between seminal plasma-free thiol levels and sperm concentration and progressive motility (r = 0.383, = 0.008 and r = 0.333, = 0.022, respectively). In addition, a significant positive correlation was found between MDA levels in seminal plasma and sperm concentration (r = 0.314, = 0.031). This study supports that seminal plasma-free thiols may be promising as local OS biomarkers. No associations were observed between local and systemic OS biomarker concentrations.
全球约10%的夫妇存在不孕问题，其中20%-30%的病例中男性因素是唯一的不孕原因。氧化应激（OS）被认为与男性不育的病理生理学有关。在精子中，OS会导致细胞膜受损，进而破坏DNA完整性并降低活力。公认的OS生物标志物包括游离巯基和丙二醛（MDA），二者均代表反应性物种相互作用组（RSI）的不同成分。这项探索性研究旨在调查精液中游离巯基和MDA水平与世界卫生组织（WHO）定义的精液参数之间的关系，以确定这些标志物是否足以定义局部OS状态。此外，本研究通过比较精液中游离巯基（R-SH，巯基，代表细胞外氧化还原状态）和MDA（脂质过氧化产物）水平与血清中测量值，来研究全身和局部OS状态之间是否存在关联。对50名寻求生育治疗的夫妇中的男性（18-55岁）进行了血清和精液中游离巯基及MDA的检测。精液中游离巯基水平与精子浓度和前向运动能力之间存在显著正相关（分别为r = 0.383，P = 0.008和r = 0.333，P = 0.022）。此外，精液中MDA水平与精子浓度之间也存在显著正相关（r = 0.314，P = 0.031）。本研究支持精液中游离巯基有望作为局部OS生物标志物。未观察到局部和全身OS生物标志物浓度之间存在关联。
