College of Forestry, Henan Agricultural University, Zhengzhou 450002, China.
Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China.
Genes (Basel). 2023 Jul 27;14(8):1540. doi: 10.3390/genes14081540.
As significant Ca sensors, calmodulin (CaM) and calmodulin-like proteins (CML), have been associated with a variety of environmental conditions in plants. However, whether CaMs/CMLs are related to the stress of phytoplasma infection has not been reported in . In the current study, 5 and 58 were detected through a genome-wide investigation. The number of EF-hand motifs in all PfCaMs/CMLs varied. Bioinformatics analyses, including protein characteristics, conserved domain, gene structure, -elements, evolutionary relationship, collinearity, chromosomal location, post-translation modification site, subcellular localization and expression pattern analyses, represented the conservation and divergence of /. Furthermore, some / might be involved in plants' reaction to phytoplasma infection and exogenous calcium therapy, indicating these genes may play a role in abiotic as well as biotic stress responses. In addition, subcellular localization analysis showed that PfCML10 was located in the cell membrane and nucleus. In summary, these findings establish a stronger platform for their subsequent functional investigation in trees and further characterize their roles in Paulownia witches' broom (PaWB) occurrence.
作为重要的钙传感器，钙调蛋白（CaM）和钙调蛋白样蛋白（CML）与植物的各种环境条件有关。然而，在 中，CaMs/CMLs 是否与植原体感染的胁迫有关尚未报道。在本研究中，通过全基因组调查检测到 5 个 PfCaMs 和 58 个 CML。所有 PfCaMs/CMLs 中的 EF 手模体的数量不同。生物信息学分析，包括蛋白质特征、保守结构域、基因结构、调控元件、进化关系、共线性、染色体定位、翻译后修饰位点、亚细胞定位和表达模式分析，代表了 的保守性和分化性。此外，一些 可能参与植物对植原体感染和外源钙治疗的反应，表明这些基因可能在非生物和生物胁迫反应中发挥作用。此外，亚细胞定位分析表明 PfCML10 位于细胞膜和细胞核中。总之，这些发现为它们在杨树中的后续功能研究建立了一个更强的平台，并进一步表征了它们在泡桐丛枝病（PaWB）发生中的作用。
