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硝酸盐转运蛋白 2 () 家族基因、鉴定及其在非生物胁迫耐受性中的潜在功能。

Nitrate Transporter 2 () Family Genes, Identification, and Their Potential Functions in Abiotic Stress Tolerance.

机构信息

College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271000, China.

出版信息

Genes (Basel). 2023 Jul 31;14(8):1564. doi: 10.3390/genes14081564.

Abstract

Nitrate transporter 2 (NRT2) proteins play vital roles in both nitrate (NO) uptake and translocation as well as abiotic stress responses in plants. However, little is known about the gene family in . In this study, 14 were identified in the genome. The family members contain the PLN00028 and MATE_like superfamily domains. -element analysis indicated that regulatory elements related to stress responses are abundant in the promoter sequences of genes. expression was increased after drought stress, and and expression were significantly upregulated after salt stress. Furthermore, protein interaction predictions suggested that homologs of BrNRT2.3, BrNRT2.1, and BrNRT2.8 in may interact with the known stress-regulating proteins AtNRT1.1, AtNRT1.5, and AtNRT1.8. In conclusion, we suggest that , and have the greatest potential for inducing abiotic stress tolerance. Our findings will aid future studies of the biological functions of family genes.

摘要

硝酸盐转运蛋白 2(NRT2)在植物硝酸盐(NO)吸收和转运以及非生物胁迫响应中发挥着重要作用。然而,人们对. 中的基因家族知之甚少。在这项研究中,在. 基因组中鉴定出 14 个。这些家族成员包含 PLN00028 和 MATE_like 超家族结构域。启动子序列分析表明,与胁迫反应相关的调控元件在. 基因的启动子序列中丰富存在。干旱胁迫后. 表达量增加,盐胁迫后. 和. 表达量显著上调。此外,蛋白质相互作用预测表明,. 中的 BrNRT2.3、BrNRT2.1 和 BrNRT2.8 同源物可能与已知的应激调节蛋白 AtNRT1.1、AtNRT1.5 和 AtNRT1.8 相互作用。综上所述,我们认为. 、. 和. 最有可能诱导非生物胁迫耐受性。我们的研究结果将有助于进一步研究. 家族基因的生物学功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a77/10454591/c4fa05d4054a/genes-14-01564-g001.jpg

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