Genome-Wide Identification and Expression Analysis of Genes Reveal Their Potential Roles in Cold and Drought Stress Tolerance in .

The gene family is pivotal in regulating plant growth, development, and stress responses. However, the role of in remains unclear. This study identifies 20 genes distributed across 6 chromosomes, categorized into 4 subfamilies. Analysis of their promoter sequences reveals multiple stress-related elements, including those responding to drought, low temperature, methyl jasmonate (MeJA), and gibberellin (GA). Gene expression profiling demonstrates wide expression of in callus, stem, silique, and flower tissues. Notably, expression significantly decreases under low-temperature treatment, while and show increased expression during drought stress, followed by a decrease. Protein interaction predictions suggest that homolog, , can interact with DES1, a known stress-regulating protein. Additionally, microRNA172 targeting is upregulated under cold tolerance. These findings underscore the vital role of in abiotic stress tolerance. Specifically, , and show great potential for regulating abiotic stress. This study contributes to understanding the function of and provides valuable insights for studying abiotic stress in .