Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Goleva Street 13, 614081 Perm, Russia.
Skolkovo Institute of Science and Technology, 121205 Moscow, Russia.
Int J Mol Sci. 2023 Aug 10;24(16):12636. doi: 10.3390/ijms241612636.
Commensal bacteriocin-producing are of interest for possible use as probiotics to selectively control the spread of pathogenic bacteria. Here, we evaluated the biosafety and efficacy of two new bacteriocin-producing strains, Q5 (VKM B-3706D) and C41 (VKM B-3707D), isolated from healthy farm animals. The genomes of both strains were sequenced, and genes responsible for the antagonistic and colonization abilities of each strain were identified. In vitro studies have shown that both strains were medium-adhesive and demonstrated antagonistic activity against most enteropathogens tested. Oral administration of 5 × 10 to 5 × 10 colony-forming units of both strains to rats with drinking water did not cause any disease symptoms or side effects. Short-term (5 days) oral administration of both strains protected rats from colonization and pathogenic effects of a toxigenic beta-lactam-resistant strain of C55 and helped preserve intestinal homeostasis. Taken together, these in silico, in vitro, and in vivo data indicate that both strains (and especially Q5) can be potentially used for the prevention of colibacillosis in farm animals.
产共生细菌素的 是一种很有前途的益生菌，可用于选择性控制致病菌的传播。在这里，我们评估了两种新的产细菌素的 菌株 Q5（VKM B-3706D）和 C41（VKM B-3707D）的生物安全性和功效，它们是从健康的农场动物中分离出来的。对这两个菌株的基因组进行了测序，并鉴定了每个菌株的拮抗和定植能力的相关基因。体外研究表明，这两种菌株都是中等粘附性的，并对大多数测试的肠道病原体表现出拮抗活性。向大鼠饮用水中口服 5×10 至 5×10 个菌落形成单位的两种菌株，不会引起任何疾病症状或副作用。两种菌株的短期（5 天）口服给药可保护大鼠免受产毒、耐β-内酰胺的 C55 菌株的定植和致病影响，并有助于维持肠道内稳态。综上所述，这些计算机模拟、体外和体内数据表明，两种菌株（特别是 Q5）可用于预防农场动物的大肠杆菌病。
