Physical Chemical Department, Institute of Marine Research (INMAR), International Campus of Excellence of the Sea (CEIMAR), Faculty of Marine and Environmental Sciences, University of Cadiz, 11510 Puerto Real, Spain.
Center for Research and Innovation in Biodiversity and Climate Change (ADAPTIA), Faculty of Engineering, University Simón Bolívar, Barranquilla 59-65, Colombia.
Int J Mol Sci. 2023 Aug 14;24(16):12798. doi: 10.3390/ijms241612798.
Marine ecosystems are among the richest in terms of biodiversity, and at present, still remain largely unknown today. In the molecular biology era, several analyses have been conducted to unravel the biological processes in this ecosystem. These systems have provided biotechnological solutions to current problems, including the treatment of diseases, as well as for the development of new biotechnological tools with applications in biomedicine and/or agri-food. In addition, in the context of climate change and global warming, these studies become even more necessary for the development of molecular tools that allow a reliable follow-up of this situation to anticipate alterations and responses of bioindicator species and to create a database to prevent and predict the environmental and climatic changes before the damage is irreversible. Proteomics approaches have revealed their potential use to obtain the set of biological effectors that lead to the real biological station on a specific stage, the proteins. In addition, proteomics-based algorithms have allowed the discovery of proteins with new potential biotechnological applications from proteome data through "applied proteomics". In this project, the first proteome analysis of the sea anemone, , and its symbiont has been developed. These organisms present a wide distribution sea ecosystem. In Spain, it is accepted as a fishing and aquaculture species. Moreover, has a symbiotic relation with autotroph Dinoflagellates, spp., that allows the study of its relation at the molecular level. For the first characterization of proteome, three independent biological replicates were used, and proteins were extracted and analyzed by LC-MS/MS, allowing the quantification of 325 proteins, 81 from spp. proteins and 244 from proteins. These proteins were subjected to gene ontology categorization by Cellular Component, Molecular Function and Biological Process. These analyzes have allowed the identification of biomarkers of gene expression as potential powerful emerging diagnostic tools to identify and characterize the molecular drivers of climate change stresses and improve monitoring techniques. In addition, through the application of novel algorithms for the detection of bioactive compounds based on the analysis of molecules of marine origin, the proteome has allowed the identification of proteins with potential applications in the fields of biomedicine and agri-food.
海洋生态系统在生物多样性方面是最丰富的，而目前这些生态系统在很大程度上仍然未知。在分子生物学时代，已经进行了几项分析，以揭示这个生态系统中的生物过程。这些系统为解决当前的问题提供了生物技术解决方案，包括治疗疾病，以及开发新的生物技术工具，应用于生物医学和/或农业食品。此外，在气候变化和全球变暖的背景下，这些研究对于开发分子工具变得更加必要，这些工具可以可靠地跟踪这种情况，预测生物指示剂物种的变化和反应，并创建一个数据库，以防止和预测环境和气候的变化，以免造成不可逆转的损害。蛋白质组学方法已经证明了它们在获得导致特定阶段真实生物状态的生物效应子组方面的潜在用途，即蛋白质。此外，基于蛋白质组学的算法通过“应用蛋白质组学”允许从蛋白质组数据中发现具有新的潜在生物技术应用的蛋白质。在这个项目中，已经开发了海葵和它的共生体的第一个蛋白质组分析。这些生物在海洋生态系统中分布广泛。在西班牙，它被认为是一种渔业和水产养殖物种。此外，它与自养甲藻属，种，具有共生关系，这允许在分子水平上研究它的关系。为了首次对 蛋白质组进行表征，使用了三个独立的生物学重复，通过 LC-MS/MS 提取和分析蛋白质，允许定量 325 种蛋白质，其中 81 种来自 种，244 种来自 种蛋白质。这些蛋白质被进行基因本体论分类，分为细胞成分、分子功能和生物过程。这些分析允许识别基因表达的生物标志物，作为识别和表征气候变化应激的分子驱动因素并改善监测技术的潜在强大新兴诊断工具。此外，通过应用基于海洋来源分子分析的新型算法来检测生物活性化合物，蛋白质组允许鉴定具有在生物医学和农业食品领域应用潜力的蛋白质。
