Becerra-Amezcua Mayra P, Matadamas-Guzmán Fernando M, Hernández-Orihuela Lorena, Guerrero-Legarreta Isabel, Guzmán-García Xochitl
Department of Hydrobiology, Division of Biological and Health Sciences, Ecotoxicology Laboratory, Universidad Autónoma Metropolitana, Iztapalapa Unit, Mexico City C. P. 09340, Mexico.
Postgraduate in Energy and Environment, Basic Sciences and Engineering Division, Universidad Autónoma Metropolitana, Iztapalapa Unit, Mexico City C. P. 09340, Mexico.
J Proteome Res. 2025 Feb 7;24(2):742-749. doi: 10.1021/acs.jproteome.4c00780. Epub 2025 Jan 27.
Sea anemones play a crucial role in marine ecosystems. Recent studies have highlighted their physiological and ecological responses to thermal stress. Therefore, our objective was to perform a proteomic analysis of sea anemones in the Gulf of Mexico, subjected to thermal stress, to understand whether these organisms activate specific processes to resist increased temperature. We submitted one group of sea anemones to variable temperatures (26 to 32 °C) and another group to a constant temperature (28 °C) for 1.5 months. Then we subjected them to thermal stress (32 °C) for 2 weeks. We evaluated the enzymatic activity and proteome in the columns and tentacles. The main effect of the temperature regime change is a reduction in mass. Also, sea anemones synthesized proteins related to the activation of the immune system and protection against temperature. We observed decreased peroxidase activity, while superoxide dismutase activity was higher only in the constant temperature group. On the basis of these data, we deduce that sea anemones are vulnerable to climate change because they stop producing toxins in their tentacles when faced with thermal stress and activate cellular responses that make them susceptible to pathogens. These responses are not sufficient to guarantee an optimal health state.
海葵在海洋生态系统中发挥着至关重要的作用。最近的研究突出了它们对热应激的生理和生态反应。因此，我们的目标是对墨西哥湾受热应激影响的海葵进行蛋白质组分析，以了解这些生物是否会激活特定过程来抵抗温度升高。我们将一组海葵置于可变温度（26至32摄氏度）下，另一组置于恒定温度（28摄氏度）下1.5个月。然后让它们经受热应激（32摄氏度）2周。我们评估了柱体和触手的酶活性和蛋白质组。温度变化的主要影响是体重减轻。此外，海葵合成了与免疫系统激活和抵御温度相关的蛋白质。我们观察到过氧化物酶活性降低，而超氧化物歧化酶活性仅在恒温组中较高。基于这些数据，我们推断海葵易受气候变化影响，因为它们在面临热应激时会停止在触手中产生毒素，并激活使其易受病原体感染的细胞反应。这些反应不足以保证最佳健康状态。
