Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40-007 Katowice, Poland.
Department of Medicine, Division of Renal Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Int J Mol Sci. 2023 Aug 15;24(16):12826. doi: 10.3390/ijms241612826.
The use of nanoparticles like graphene oxide (GO) in nanocomposite industries is growing very fast. There is a strong concern that GO can enter the environment and become nanopollutatnt. Environmental pollutants' exposure usually relates to low concentrations but may last for a long time and impact following generations. Attention should be paid to the effects of nanoparticles, especially on the DNA stability passed on to the offspring. We investigated the multigenerational effects on two strains (wild and long-lived) of house cricket intoxicated with low GO concentrations over five generations, followed by one recovery generation. Our investigation focused on oxidative stress parameters, specifically AP sites (apurinic/apyrimidinic sites) and 8-OHdG (8-hydroxy-2'-deoxyguanosine), and examined the global DNA methylation pattern. Five intoxicated generations were able to overcome the oxidative stress, showing that relatively low doses of GO have a moderate effect on the house cricket (8-OHdG and AP sites). The last recovery generation that experienced a transition from contaminated to uncontaminated food presented greater DNA damage. The pattern of DNA methylation was comparable in every generation, suggesting that other epigenetic mechanisms might be involved.
纳米复合材料行业中氧化石墨烯（GO）等纳米颗粒的使用发展非常迅速。人们强烈担心 GO 可能会进入环境并成为纳米污染物。环境污染物的暴露通常与低浓度有关，但可能持续很长时间，并影响后代。应注意纳米颗粒的影响，特别是对传递给后代的 DNA 稳定性的影响。我们研究了经过五代低 GO 浓度处理的两种品系（野生型和长寿型）家蟋蟀的多代效应，随后进行了一代恢复。我们的研究集中在氧化应激参数上，特别是 AP 位点（无嘌呤/无嘧啶位点）和 8-OHdG（8-羟基-2'-脱氧鸟苷），并检查了全球 DNA 甲基化模式。五个受污染的世代能够克服氧化应激，表明相对较低剂量的 GO 对家蟋蟀（8-OHdG 和 AP 位点）有中度影响。最后一代经历了从受污染到未受污染食物的过渡，表现出更大的 DNA 损伤。每个世代的 DNA 甲基化模式都相似，这表明可能涉及其他表观遗传机制。
