Tu Qinghui, Deng Jianhao, Di Miaomiao, Lin Xiaorong, Chen Zhongzheng, Li Bin, Tian Ling, Zhang Yuanyuan
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Chemosphere. 2023 Jul;330:138724. doi: 10.1016/j.chemosphere.2023.138724. Epub 2023 Apr 18.
Micro-nanoplastics have become a new type of pollutant worldwide and have attracted widespread attention for their potential toxicity. However, the toxicity of polystyrene nanoplastics (PS-NPs) under continuous exposure of multi-generations is still unclear. In the present study, Drosophila melanogaster was selected as an in vivo biological model to investigate the reproductive toxicity and underlying mechanism induced by PS-NPs (100 nm; 1, 10, 50, and 100 mg L) after continuous exposure of five generations. The results showed that PS-NPs accumulated in the crop, gut and ovaries after 5 d of exposure. It was also observed that the number of egg production and eclosion rate decreased significantly (P < 0.05) accompanied by delayed development during continuous exposure PS-NPs of multi-generations. Further analysis revealed that the degree of apoptosis and necrosis of oocytes in the F5 generation increased with the increasing exposure dose. To elucidate the underlying toxicity mechanism mediated by PS-NPs, transcriptomic analysis was performed on the ovaries of the F5 generation. The results showed that there were 102 and 208 differentially expressed genes (DEGs) in the 1 mg L and 100 mg L PS-NPs treatment groups, respectively, compared with the control group. The transcriptome analysis further detected the KEGG pathway with significant enrichment of DEGs, revealing obvious reproductive toxicity at the molecular level. In conclusion, this research not only highlighted the negative physiological effects of multi-generational exposure to PS-NPs on Drosophila melanogaster, but also explored potential mechanisms by transcriptomic analysis to better understand reproductive toxicity induced by multi-generational exposure.
微纳塑料已成为全球新型污染物，因其潜在毒性而备受广泛关注。然而，聚苯乙烯纳米塑料（PS-NPs）在多代连续暴露下的毒性仍不明确。在本研究中，选择黑腹果蝇作为体内生物学模型，以研究连续五代暴露于PS-NPs（100纳米；1、10、50和100毫克/升）后诱导的生殖毒性及潜在机制。结果显示，暴露5天后PS-NPs在嗉囊、肠道和卵巢中积累。还观察到，在多代连续暴露于PS-NPs期间，产卵数和羽化率显著降低（P<0.05），同时发育延迟。进一步分析表明，F5代卵母细胞的凋亡和坏死程度随暴露剂量增加而增加。为阐明PS-NPs介导的潜在毒性机制，对F5代卵巢进行了转录组分析。结果显示，与对照组相比，1毫克/升和100毫克/升PS-NPs处理组分别有102个和208个差异表达基因（DEGs）。转录组分析进一步检测到KEGG通路中DEGs显著富集，揭示了分子水平上明显的生殖毒性。总之，本研究不仅突出了多代暴露于PS-NPs对黑腹果蝇的负面生理影响，还通过转录组分析探索了潜在机制，以更好地理解多代暴露诱导的生殖毒性。
