Yermagambetova Moldir, Almerekova Shyryn, Turginov Orzimat, Sultangaziev Ormon, Abugalieva Saule, Turuspekov Yerlan
Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan.
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
Plants (Basel). 2023 Aug 16;12(16):2961. doi: 10.3390/plants12162961.
Kom. is a species that grows widely in the mountain ranges from Central Asia to Oman. It is an important tree for the formation of shrub-forest massifs in mountainous areas and for draining and fixing soils from middle to high altitudes. A comprehensive study of the species' genetic diversity and population structure is a basic approach to understanding the current status of resources for the development of future conservation strategies. Samples from 15 populations of were collected from the mountain ranges of Uzbekistan, Kyrgyzstan, and Kazakhstan. The genetic diversity and population structure of 15 Central Asian populations of were assessed using 11 polymorphic simple sequence repeat (SSR) markers. Genetic diversity parameters, including the number of alleles (na), the effective number of alleles (ne), Shannon's information index (I), the percentage of polymorphic loci (PPL), Nei's genetic diversity index (Nei), principal coordinate analysis (PCoA), etc., were evaluated. The analysis of 15 populations based on 11 polymorphic SSRs detected 35 alleles. The average PIC value was 0.432, and the highest value (0.662) was found in the JT_40 marker. Nei's genetic diversity index for the populations was 0.450, ranging from 0.407 (population 14) to 0.566 (population 4). The analysis of molecular variance (AMOVA) showed that 90.3% of total genetic variation is distributed within the population. Using the alleles of all the populations, the gene flow (Nm) was found to be 4.654. Population structure analysis revealed poor clustering in the studied populations and confirmed our AMOVA results. The output of this work can be efficiently used for the maintenance of the species across the Central Asian region.
Kom.是一种广泛生长于从中亚到阿曼山脉的物种。它是山区灌丛林地形成以及中高海拔地区土壤排水和固定的重要树木。对该物种的遗传多样性和种群结构进行全面研究是了解当前资源状况以制定未来保护策略的基本方法。从乌兹别克斯坦、吉尔吉斯斯坦和哈萨克斯坦的山脉采集了该物种15个种群的样本。使用11个多态性简单序列重复（SSR）标记评估了中亚地区15个种群的遗传多样性和种群结构。评估了包括等位基因数（na）、有效等位基因数（ne）、香农信息指数（I）、多态位点百分比（PPL）、内氏遗传多样性指数（Nei）、主坐标分析（PCoA）等遗传多样性参数。基于11个多态性SSR对15个种群的分析检测到35个等位基因。平均PIC值为0.432，在JT_40标记中发现最高值（0.662）。该种群的内氏遗传多样性指数为0.450，范围从0.407（种群14）到0.566（种群4）。分子方差分析（AMOVA）表明，90.3%的总遗传变异分布在种群内部。利用所有种群的等位基因，发现基因流（Nm）为4.654。种群结构分析显示所研究种群的聚类较差，并证实了我们的AMOVA结果。这项工作的成果可有效地用于中亚地区该物种的保护。
