Plant height variation and genetic diversity between (Schlecht.) YY Yao and Batsch based on using SSR markers in East Kazakhstan.

BACKGROUND

Genetic differences between isolated endemic populations of plant species and those with widely known twin species are relevant for conserving the biological diversity of our planet's flora. (Schlecht.) YY Yao is an endangered and endemic species of shrub almond from central Asia. Few studies have explored this species, which is closely related and morphologically similar to the well-known Batsch. In this article, we present a comparative analysis of studies of three populations and one close population of in Eastern Kazakhstan in order to determine the particular geographic mutual replacement of the two species.

METHODS

The populations were collected from different ecological niches, including one steppe population near Ust-Kamenogorsk () and three populations () in the mountainous area. Estimation of plant height using a -test suggested a statistically significant difference between the populations and the two species ( < 0.0001). DNA simple sequence repeat (SSR) markers were applied to study the two species' genetic diversity and population structure.

RESULTS

A total of 19 polymorphic SSR loci were analyzed, and the results showed that the population collected in mountainous areas had a lower variation level than steppe populations. The highest level of Nei's genetic diversity index was demonstrated in the 4-UK population (0.622) of . The lowest was recorded in population 3-KA (0.461) of , collected at the highest altitude of the four populations (2,086 meters above sea level). The total genetic variation of was distributed 73% within populations and 27% between populations. STRUCTURE results showed that two morphologically similar species diverged starting at step  = 3, with limited population mixing. The results confirmed the morphological and genetic differences between and and described the level of genetic variation for . The study's results proved that the steppe zone and mountain altitude factor between and isolated mountain samples of .