Wang Yidan, Cao Ruifang, Yang Lu, Duan Xiaoyu, Zhang Can, Yu Xuejing, Ye Xueling
Collage of Horticulture, Shenyang Agricultural University, 120 Dongling Road Shenhe District, Shenyang 110866, China.
Plants (Basel). 2023 Aug 16;12(16):2963. doi: 10.3390/plants12162963.
Cucumbers ( L.) are a global popular vegetable and are widely planted worldwide. However, cucumbers are susceptible to various infectious diseases such as Fusarium and Verticillium wilt, downy and powdery mildew, and bacterial soft rot, which results in substantial economic losses. Grafting is an effective approach widely used to control these diseases. The present study investigated the role of wax and the phenylpropanoid biosynthesis pathway in black-seed pumpkin rootstock-grafted cucumbers. Our results showed that grafted cucumbers had a significantly higher cuticular wax contents on the fruit surface than that of self-rooted cucumbers at all stages observed. A total of 1132 differently expressed genes (DEGs) were detected in grafted cucumbers compared with self-rooted cucumbers. Pathway enrichment analysis revealed that phenylpropanoid biosynthesis, phenylalanine metabolism, plant circadian rhythm, zeatin biosynthesis, and diterpenoid biosynthesis were significantly enriched. In this study, 1 and 13 genes involved in wax biosynthesis and the phenylpropanoid biosynthesis pathway, respectively, were up-regulated in grafted cucumbers. Our data indicated that the up-regulated genes in the wax and phenylpropanoid biosynthesis pathways may contribute to disease resistance in rootstock-grafted cucumbers, which provides promising targets for enhancing disease resistance in cucumbers by genetic manipulation.
黄瓜（Cucumis sativus L.）是一种全球广受欢迎的蔬菜，在世界各地广泛种植。然而，黄瓜易受多种传染病影响，如镰刀菌枯萎病、黄萎病、霜霉病、白粉病和细菌性软腐病，这导致了巨大的经济损失。嫁接是一种广泛用于控制这些病害的有效方法。本研究调查了蜡质和苯丙烷生物合成途径在黑籽南瓜砧木嫁接黄瓜中的作用。我们的结果表明，在所有观察阶段，嫁接黄瓜果实表面的角质蜡含量均显著高于自根黄瓜。与自根黄瓜相比，嫁接黄瓜中共检测到1132个差异表达基因（DEGs）。通路富集分析表明，苯丙烷生物合成、苯丙氨酸代谢、植物昼夜节律、玉米素生物合成和二萜生物合成显著富集。在本研究中，嫁接黄瓜中分别有1个和13个参与蜡质生物合成和苯丙烷生物合成途径的基因上调。我们的数据表明，蜡质和苯丙烷生物合成途径中的上调基因可能有助于砧木嫁接黄瓜的抗病性，这为通过基因操作提高黄瓜抗病性提供了有希望的靶点。
