Lee Eun-Song, Kim Yong-Il, Lee Jeong-Hoon, Kim Yong-Goo, Han Kyung-Sook, Yoon Young-Ho, Cho Byoung-Ok, Park Kyungtae, Lee Hamin, Cho Ju-Sung
Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumseong 27709, Republic of Korea.
Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
Plants (Basel). 2023 Aug 17;12(16):2975. doi: 10.3390/plants12162975.
Recently, there has been a growing interest in the consumption of plant-based foods such as vegetables and grains for the purpose of disease prevention and treatment. Adlay seeds contain physiologically active substances, including coixol, coixenolide, and lactams. In this study, adlay sprouts were cultivated and harvested at various time points, specifically at 3, 5, 7, 9, and 11 days after sowing. The antioxidant activity of the extracts was evaluated using assays such as DPPH radical scavenging, ABTS radical scavenging, reducing power, and total polyphenol contents. The toxicity of the extracts was assessed using cell culture and the WST-1 assay. The aboveground components of the sprouts demonstrated a significant increase in length, ranging from 2.75 cm to 21.87 cm, weight, ranging from 0.05 g to 0.32 g, and biomass, ranging from 161.4 g to 1319.1 g, as the number of days after sowing advanced, reaching its peak coixol content of 39.38 mg/g on the third day after sowing. Notably, the antioxidant enzyme activity was highest between the third and fifth days after sowing. Regarding anti-inflammatory activity, the inhibition of cyclooxygenase 2 (COX-2) expression was most prominent in samples harvested from the ninth to eleventh days after sowing, corresponding to the later stage of growth. While the overall production mass increased with the number of days after sowing, considering factors such as yield increase index per unit area, turnover rate, and antioxidant activity, harvesting at the early growth stage, specifically between the fifth and seventh days after sowing, was found to be economically advantageous. Thus, the quality, antioxidant capacity, and anti-inflammatory activity of adlay sprouts varied depending on the harvest time, highlighting the importance of determining the appropriate harvest time based on the production objectives. This study demonstrates the changes in the growth and quality of adlay sprouts in relation to the harvest time, emphasizing the potential for developing a market for adlay sprouts as a new food product.
最近，人们对食用蔬菜和谷物等植物性食物以预防和治疗疾病的兴趣日益浓厚。薏仁种子含有生理活性物质，包括薏苡仁醇、薏苡仁酯和内酰胺。在本研究中，在不同时间点种植并收获了薏仁芽苗，具体为播种后3天、5天、7天、9天和11天。使用DPPH自由基清除、ABTS自由基清除、还原能力和总多酚含量等测定方法评估提取物的抗氧化活性。使用细胞培养和WST-1测定法评估提取物的毒性。随着播种后天数的增加，芽苗地上部分的长度从2.75厘米显著增加到21.87厘米，重量从0.05克增加到0.32克，生物量从161.4克增加到1319.1克，播种后第三天达到其最高薏苡仁醇含量39.38毫克/克。值得注意的是，抗氧化酶活性在播种后第三天至第五天之间最高。关于抗炎活性，播种后第九天至第十一天收获的样品中环氧合酶2（COX-2）表达的抑制最为显著，这对应于生长后期。虽然总产量随着播种后天数的增加而增加，但考虑到单位面积产量增加指数、周转率和抗氧化活性等因素，发现在生长早期，即播种后第五天至第七天之间收获在经济上具有优势。因此，薏仁芽苗的质量、抗氧化能力和抗炎活性因收获时间而异，突出了根据生产目标确定合适收获时间的重要性。本研究展示了薏仁芽苗生长和质量与收获时间的关系变化，强调了开发薏仁芽苗作为新食品市场的潜力。
