基因组分析I：在快速发现与基因座连锁的DNA标记中的遗传单位和遗传选择
Genomic analysis I: inheritance units and genetic selection in the rapid discovery of locus linked DNA markers.
作者信息
Sanda A I, Ford J P
出版信息
Nucleic Acids Res. 1986 Sep 25;14(18):7265-83. doi: 10.1093/nar/14.18.7265.
We propose, and test using a Monte-Carlo analysis (a computer-based numerical analysis using a random number generator), a novel and efficient method to obtain sets of DNA markers linked to any inherited genetic locus. The method consists of a targeted search that is based on the common inheritance among members of an outbred pedigree, of discrete chromosome lengths, which we call inheritance units, to obtain DNA markers linked to the locus. In cases where two individuals inherit the same trait through two different lines of descent from a common ancestor, the set of inheritance units in each of the two genomes includes an inheritance unit that is identical in both individuals for a substantial distance on both sides of the DNA sequence which confers the trait. The power of the technique derives from the genetic selection that reduces the size and number of the inheritance units as the generational distance between the two individuals being compared increases.
我们提出并使用蒙特卡洛分析（一种基于随机数生成器的计算机数值分析方法）测试了一种新颖且高效的方法，以获取与任何遗传基因座相连的DNA标记集。该方法包括基于远交系谱成员间共同遗传的有针对性搜索，搜索离散的染色体长度，我们将其称为遗传单位，以获得与该基因座相连的DNA标记。在两个个体通过来自共同祖先的两条不同世系继承相同性状的情况下，两个基因组中每个基因组的遗传单位集都包括一个遗传单位，该遗传单位在赋予该性状的DNA序列两侧相当长的距离内，在两个个体中是相同的。该技术的强大之处源于遗传选择，随着被比较的两个个体之间的世代距离增加，遗传单位的大小和数量会减少。
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