Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Abderrahmane Mira, Bejaia 06000, Algeria.
Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University Setif 1, Setif 19000, Algeria.
J Pharm Biomed Anal. 2023 Oct 25;235:115673. doi: 10.1016/j.jpba.2023.115673. Epub 2023 Aug 20.
Hertia cheirifolia L. is a medicinal plant that has been used for a long time in folk Mediterranean medicine. The aim of the present study was to analyze and compare the phenolic profile and the antioxidant potential of organic fractions from H. cheirifolia extracts. Crude methanolic extracts were firstly prepared from the different parts of the plant. Then four different organic fractions were obtained by fractioning each extract, using different solvents with increasing polarity (hexane, chloroform, and ethyl acetate). The Phenolic content was analyzed using a UV-Vis colorimetric methods followed by a qualitative and quantitative analysis by high performance liquid chromatography-diode array detector (HPLC-DAD) system. After that, the antioxidant potential of the different organic fractions was evaluated using DPPH and ABTS free radical scavenging assays, reducing power of iron (FRAP) and inhibition of β-carotene oxidation tests. Our results revealed that ethyl acetate fractions (EA) contained the highest content of total phenolics (100-250 mg GAE/g). Indeed, the ethyl acetate fraction from the flower extract (EA-F) displayed the lowest IC values for the scavenging of DPPH and ABTS free radicals (38.83 ± 0.34 µg/ml and 23.76 ± 0.11 µg/ml, respectively). Also, the strongest iron reducing power (2628.87 ± 16.47 µmol FeEq/ml) and the best rate of inhibition of the β-carotene oxidation (58.91 ± 5.79 %) were recorded. In sum, the present study suggests that, the organic fractions from H. cherifolia are potential natural antioxidants and this is probably related to their phenolics content and structure.
赫蒂奇叶菊（Hertia cheirifolia L.）是一种药用植物，在民间地中海医学中已经使用了很长时间。本研究的目的是分析和比较赫蒂奇叶菊提取物的有机部分的酚类成分和抗氧化潜力。首先从植物的不同部位制备粗甲醇提取物。然后，通过用具有增加极性的不同溶剂（己烷、氯仿和乙酸乙酯）对每个提取物进行分馏，获得四种不同的有机部分。使用紫外可见分光光度法分析酚含量，然后通过高效液相色谱-二极管阵列检测器（HPLC-DAD）系统进行定性和定量分析。之后，使用 DPPH 和 ABTS 自由基清除试验、铁还原能力（FRAP）和β-胡萝卜素氧化抑制试验评估不同有机部分的抗氧化潜力。我们的结果表明，乙酸乙酯部分（EA）含有最高的总酚含量（100-250mg GAE/g）。事实上，花提取物的乙酸乙酯部分（EA-F）对 DPPH 和 ABTS 自由基的清除表现出最低的 IC 值（分别为 38.83±0.34μg/ml 和 23.76±0.11μg/ml）。此外，还记录了最强的铁还原能力（2628.87±16.47μmol FeEq/ml）和β-胡萝卜素氧化抑制率最高（58.91±5.79%）。总之，本研究表明，赫蒂奇叶菊的有机部分是潜在的天然抗氧化剂，这可能与其酚类成分和结构有关。
