Benabderrahmane Wassila, Fadel Hamza, Sekhara Ines, Mennai Imad, Kadi Imed Eddine, Helal Mahmoud, Sami Rokayya, Abo-Dief Hala M, Bedaiwi Ruqaiah I, Alanazi Mohammad A, Al-Harthi Helal F, Kadi Roqayah H, Abushal Suzan A, Albishi Tasahil S, Qumsani Alaa T, Qari Sameer H
Department of Chemistry, Faculty of Sciences, University of 20 Août 1955-Skikda PO Box 26 El-Hadaiek Road Algeria
Research Unit Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses, University of the Mentouri Brothers Constantine1, Aïn El Bey Road 25000 Constantine Algeria
RSC Adv. 2024 Jul 17;14(31):22548-22559. doi: 10.1039/d4ra03578j. eCollection 2024 Jul 12.
The genus , which belongs to the Asteraceae family, is a flowering genus with 12 species found in Africa, North and South. Among the species present in Algeria, L. is distributed in the eastern regions of Algeria. The aim of this study is to evaluate its phytochemical composition with following pharmacological assessments: the antioxidant, antibacterial, and antifungal activities of L. essential oil (EO). GC-MS analysis was used to analyze the chemical constituents of essential oil. The antioxidant capacity was assessed using DPPH, FRAP, and HO tests. The EO was also tested for its ability to inhibit six strains of microorganisms, including two Gram (+) and four Gram (-) strains. The antifungal activity was tested by analyzing the effect of the EO on the mycelial growth of f.sp. (FOL) fungi. Results showed that primary volatile components were α-pinene (32.59%), 2-(1-cyclopent-1-enyl-1-methylethyl) cyclopentanone (14.62%), (-)-germacrene D (11.37%), and bakkenolide A (9.57%). EO showed inhibitory effects against DPPH, HO, and FRAP (IC = 0.34 ± 0.1, 0.053 ± 0.1, and 0.047 ± 0.01 mg mL, respectively). The EO also exhibited moderate antibacterial effects against ATCC 25923 (), ATCC 49619 (), and ATCC 13048 (), as well as significant antioxidant potential and varied antifungal activity based on dosage and fungal strain. To our knowledge, no previous research has examined the antifungal capacity of oil and oil-mycelial development of the FOL relationship. To fully explore the benefits of EO, more research is necessary, along with more testing on other bacterial and fungal strains.
该属属于菊科，是一个开花植物属，在非洲、北美洲和南美洲发现了12个物种。在阿尔及利亚存在的物种中，L.分布于阿尔及利亚东部地区。本研究的目的是通过以下药理学评估来评价其植物化学成分：L.精油（EO）的抗氧化、抗菌和抗真菌活性。采用气相色谱-质谱联用（GC-MS）分析来分析精油的化学成分。使用二苯基苦味酰基自由基（DPPH）、铁离子还原抗氧化能力（FRAP）和羟基自由基（HO）测试来评估抗氧化能力。还测试了EO抑制六种微生物菌株的能力，包括两种革兰氏阳性菌和四种革兰氏阴性菌。通过分析EO对番茄叶霉菌（FOL）真菌菌丝体生长的影响来测试抗真菌活性。结果表明，主要挥发性成分是α-蒎烯（32.59%）、2-（1-环戊-1-烯基-1-甲基乙基）环戊酮（14.62%）、（-）-吉马烯D（11.37%）和木香内酯A（9.57%）。L. EO对DPPH、HO和FRAP显示出抑制作用（IC分别为0.34±0.1、0.053±0.1和0.047±0.01 mg/mL）。EO对金黄色葡萄球菌ATCC 25923、白色念珠菌ATCC 49619和枯草芽孢杆菌ATCC 13048也表现出中等抗菌作用，以及基于剂量和真菌菌株的显著抗氧化潜力和不同的抗真菌活性。据我们所知，以前没有研究考察过L.油的抗真菌能力以及油与FOL菌丝体发育的关系。为了充分探索L. EO的益处，需要进行更多的研究，以及对其他细菌和真菌菌株进行更多测试。
