Smirnova O V, Vishniakova T G, Kurabekova R M, Rozen V B
Probl Endokrinol (Mosk). 1986 Jul-Aug;32(4):66-9.
The authors have assessed the life-span of special estrogen-binding rat liver protein (SEBP) through inhibition of it by long-term administration of cyclohexymid (C1) and have examined how C1 affects the efficiency with which sex steroids influence the level of SEBP. The level of SEBP synthesis was assessed by the changes in the number of SEBP estradiol (E2)-binding sites. The long-term C1 administration in the dose of 100 micrograms suppressed SEBP synthesis by an average of 82.4% with the half-life of SEBP being about 18 hr. Testosterone propionate (TP) and 5 alpha-dihydrotestosterone, but not E2, were found able to mediate enhanced SEBP levels in the liver of ovariectomized female rats. A single 200 micrograms dose of C1 administered 30 min prior to hormone injection caused significant decreases in the efficiency of SEBP-mediated androgen activities in the liver of ovariectomized female rats, in TP-induced stimulation of the SEBP level in the liver of castrated male rats and in E2 inhibitory effect on the SEBP level in the liver of mature males. It is concluded that de novo protein synthesis is necessary for the early manifestation of all the above mentioned effects of sex hormones on the SEBP level. Based on the data regarding the duration of SEBP life and the rate with which it affects the activity of sex steroids it is supposed that in the early stages of hormonally induced manifestations there are changes in biosynthesis of regulating protein rather than in SEBP itself.
作者通过长期给予环己酰亚胺（C1）抑制特殊雌激素结合大鼠肝蛋白（SEBP），评估了其寿命，并研究了C1如何影响性类固醇影响SEBP水平的效率。通过SEBP雌二醇（E2）结合位点数量的变化评估SEBP合成水平。以100微克剂量长期给予C1可使SEBP合成平均抑制82.4%，SEBP的半衰期约为18小时。发现丙酸睾酮（TP）和5α-二氢睾酮而非E2能够介导去卵巢雌性大鼠肝脏中SEBP水平的升高。在激素注射前30分钟给予单次200微克剂量的C1，可使去卵巢雌性大鼠肝脏中SEBP介导的雄激素活性效率、TP诱导的去势雄性大鼠肝脏中SEBP水平的升高以及E2对成熟雄性大鼠肝脏中SEBP水平的抑制作用均显著降低。得出的结论是，从头合成蛋白质对于性激素对SEBP水平的上述所有影响的早期表现是必要的。基于关于SEBP寿命持续时间及其影响性类固醇活性速率的数据，推测在激素诱导表现的早期阶段，调节蛋白的生物合成发生了变化，而非SEBP本身。
