Smirnova O V, Miroshnichenko M L, Shchelkunova T A, Vishniakova T G, Smirnov A N
Probl Endokrinol (Mosk). 1983 Sep-Oct;29(5):65-70.
A new modification of specific estrogen-binding protein (SEBP) assay in the liver was used to measure the content of this protein at different stages of ontogenesis in male and female rats after administration of testosterone propionate (TP). It was shown that liver capability to form SEBP could be initially programmed by androgens. The crucial period of the SEBP-determining action of androgens in females is not restricted, since potential capability of androgens of primary induction of SEBP may be realized both neonatally and in the course of pubertation. The emergence of SEBP in the liver of males may be the result of natural pre(neo)natal androgenization. Androgens do not play any decisive role in the control of the level of induced SEBP, inasmuch the content of SEBP in the liver of non-pubertal and pubertal males does not appreciably change after TP administration but grows with pubescence. SEBP is capable of long-term existence in the absence of androgens. The presence of the ovaries produces an inhibitory effect on the level of androgen-induced SEBP and the time of its existence after TP discontinuation. Hypophysectomy does not provoke SEBP emergence in pubertal intact and ovariectomized females but prevents its induction with androgens. Adrenal- and thyroectomy produce no influence on the SEBP-programming effect of TP or promote its spontaneous appearance in the same groups of female rats.
采用一种新改良的肝脏中特异性雌激素结合蛋白（SEBP）检测方法，来测定丙酸睾酮（TP）给药后雄性和雌性大鼠个体发育不同阶段该蛋白的含量。结果表明，肝脏形成SEBP的能力最初可能由雄激素编程。雄激素对雌性大鼠SEBP的决定性作用关键期并不受限，因为雄激素对SEBP的初级诱导潜在能力在新生期和青春期过程中均可实现。雄性大鼠肝脏中SEBP的出现可能是自然产前（新生期）雄激素化的结果。雄激素在诱导SEBP水平的控制中不起任何决定性作用，因为非青春期和青春期雄性大鼠肝脏中SEBP的含量在给予TP后并无明显变化，但会随着青春期的到来而增加。SEBP在没有雄激素的情况下能够长期存在。卵巢的存在对雄激素诱导的SEBP水平及其在停用TP后的存在时间产生抑制作用。垂体切除不会促使青春期未阉割和去卵巢雌性大鼠出现SEBP，但会阻止其被雄激素诱导。肾上腺切除和甲状腺切除对TP的SEBP编程效应没有影响，也不会促进同一组雌性大鼠中SEBP的自发出现。
