Khepar Varinder, Ahuja Radha, Sidhu Anjali, Samota Mahesh K
Department of Chemistry, Punjab Agricultural University, Ludhiana, Punjab 141004, India.
Department of Soil Science, Punjab Agricultural University, Ludhiana, Punjab 141004, India.
ACS Omega. 2023 Aug 10;8(33):30231-30238. doi: 10.1021/acsomega.3c03012. eCollection 2023 Aug 22.
Physiological and biochemical mechanisms behind nanoparticle (NP)-induced seed germination by nanopriming with metal sulfide NPs are lacunae in the field of agriculture. Sonochemically synthesized aqua-dispersed ferrous sulfide NPs (FeS-NPs) and manganese sulfide NPs (MnS-NPs) were examined as nanopriming agents for physiological, pathological, and antioxidative defense parameters of rice in the present study. Under pot house conditions, in vivo nanopriming of rice seeds with FeS NPs and MnS-NPs at a concentration of 35 μg/mL for 8 h significantly improved the physiological parameters, viz., germination percentage, seed germination index, mean germination time, dry weight, and vigor index, and decreased the phytopathological parameters of nanoprimed rice seeds, viz., mortality, seed rot, and seedling blight. Stimulation of superoxide dismutase (SOD ≥ 28.16%), ascorbate peroxidase (APX ≥ 52.38%), and catalase (CAT ≥ 28.57%) enzymes in FeS-NP- and MnS-NP-nanoprimed seeds as compared to control (hydroprimed seeds) enhanced the fitness of rice seedlings. The augmented levels of Fe and Mn content in the shoots and roots of NP-treated seedlings as compared to hydroprimed seedlings confirmed the incorporation nanometals in rice seedlings as nanonutrients for effective plant growth. Inclusively, FeS-NPs and MnS-NPs were shown to be effective nanopriming agents for promoting the germination of naturally fungal infested rice seeds.
在农业领域，通过用金属硫化物纳米颗粒进行纳米引发来诱导种子萌发背后的生理和生化机制尚不清楚。在本研究中，对通过声化学合成的水分散性硫化亚铁纳米颗粒（FeS-NPs）和硫化锰纳米颗粒（MnS-NPs）作为水稻生理、病理和抗氧化防御参数的纳米引发剂进行了研究。在温室条件下，用浓度为35μg/mL的FeS NPs和MnS-NPs对水稻种子进行8小时的体内纳米引发，显著改善了生理参数，即发芽率、种子发芽指数、平均发芽时间、干重和活力指数，并降低了纳米引发水稻种子的植物病理参数，即死亡率、种子腐烂和幼苗枯萎病。与对照（水引发种子）相比，FeS-NP和MnS-NP纳米引发种子中的超氧化物歧化酶（SOD≥28.16%）、抗坏血酸过氧化物酶（APX≥52.38%）和过氧化氢酶（CAT≥28.57%）的活性增强，提高了水稻幼苗的适应性。与水引发的幼苗相比，NP处理的幼苗地上部和根部的Fe和Mn含量增加，证实了纳米金属作为纳米营养物质被水稻幼苗吸收，以促进植物有效生长。总之，FeS-NPs和MnS-NPs被证明是促进天然真菌感染水稻种子萌发的有效纳米引发剂。
