Wang Yue, Zhang Jin, Sun Jiabo, Li Guoqing, Wang Qian, Zhao Yanxia, Ma Changjian, Han Jinlong
Shandong Academy of Agricultural Sciences, Jinan, China.
Tai'an Taishan Forestry Research Institute, Tai'an, China.
Front Microbiol. 2023 Aug 10;17:1218595. doi: 10.3389/fmicb.2023.1232846. eCollection 2023.
is an understory economic plant, and its dried rhizome is a traditional Chinese medicine. The purpose of this study was to connect the quality improvement of the understory plant with specific microorganisms.
Amplicon and metabolome sequencing were conducted for interplanted under three types of trees and in the field, and the relationship between the microbiome and secondary metabolism was explored.
Principal component analysis (PCA) divided field cultivated and understory interplanted groups into two classes. A total of 95 different metabolites were found, with four expression patterns. The alpha diversity of rhizosphere bacteria and endosphere fungi in the understory interplanted group was significantly higher than that in the farmland cultivated group. There were 276 different rhizosphere microorganism genera among the four groups; however, only 33 different endosphere genera were observed, indicating that endophytic microbial diversity was relatively stable within the rhizome, especially for endosphere bacteria. Cointertia analysis (CoIA) suggested that the metabolite changes in induced by interplanting under different trees were more strongly affected by rhizosphere microorganisms than by endosphere microorganisms. In addition, the interactions between rhizosphere microorganisms and metabolites in the farmland group were weakened compared with those in the underplanted groups. Canonical correspondence analysis (CCA) showed that and had the greatest influence on the metabolites. , as a shared microbe in the rhizosphere and endosphere, had interaction effects with the largest number of microbes.
This study revealed the interactions between the microbes and metabolites in and systematically explored the mechanism underlying their correlation, which was mediated by the understory interplanting mode. This study provides feasible strategies for improving the medicinal value of by regulating microorganisms.
是一种林下经济植物，其干燥根茎为中药材。本研究旨在将林下植物的品质提升与特定微生物联系起来。
对在三种树木下间作以及在田间种植的进行扩增子和代谢组测序，探索微生物群落与次生代谢之间的关系。
主成分分析（PCA）将田间种植组和林下间作组分为两类。共发现95种不同代谢物，有四种表达模式。林下间作组根际细菌和内生真菌的α多样性显著高于农田种植组。四组之间有276个不同的根际微生物属；然而，仅观察到33个不同的内生菌属，表明根茎内内生微生物多样性相对稳定，尤其是内生细菌。冗余分析（CoIA）表明，不同树木下间作诱导的代谢物变化受根际微生物的影响大于内生微生物。此外，与林下间作组相比，农田组根际微生物与代谢物之间的相互作用减弱。典范对应分析（CCA）表明，和对代谢物影响最大。作为根际和内生菌中共有的微生物，与最多的微生物有相互作用。
本研究揭示了中微生物与代谢物之间的相互作用，并系统地探索了其相关机制，该机制由林下间作模式介导。本研究为通过调控微生物提高的药用价值提供了可行策略。
