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根际微生物组结构与功能的比较分析

A Comparative Analysis on the Structure and Function of the Rhizosphere Microbiome.

作者信息

Kui Ling, Chen Baozheng, Chen Jian, Sharifi Rouhallah, Dong Yang, Zhang Zhanjiang, Miao Jianhua

机构信息

School of Pharmacy, Jiangsu University, Zhenjiang, China.

College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.

出版信息

Front Microbiol. 2021 Jun 9;12:673512. doi: 10.3389/fmicb.2021.673512. eCollection 2021.

Abstract

, an important Chinese medicinal herb, can be mainly cultivated in two planting patterns, cropland planting (DT) and understory planting (LX). We speculate that the rhizosphere microbiome may vary in DT and LX and may play an important role in promoting the growth and health of . In the present study, culture-independent Illumina HiSeq was employed to investigate the rhizosphere bacteria and fungi under DT and LX planting patterns. Predominant phyla include , , , , and in the two planting patterns. DT has higher alpha diversity index than LX. The predominant LX-core genera include , , and the predominant DT-core genera include , , and . Total relative abundance of the disease-suppression phylum (, , and ) and the potential plant growth-promoting rhizobacteria (PGPR) were both significantly higher in LX than in DT. We also identified over-presented microbial functional traits mediating plant-microbe and microbe-microbe interactions, nutrition acquisition, and plant growth promotion in rhizosphere. Our findings provide a valuable reference for studying beneficial microbes and pathogens of planted in DT and LX.

摘要

[一种重要的中药材]主要有两种种植模式,即农田种植(DT)和林下种植(LX)。我们推测,根际微生物群在DT和LX中可能存在差异,并且可能在促进[该中药材]的生长和健康方面发挥重要作用。在本研究中,采用非培养的Illumina HiSeq技术来研究DT和LX种植模式下的根际细菌和真菌。两种种植模式下的优势菌门包括[具体菌门名称1]、[具体菌门名称2]、[具体菌门名称3]、[具体菌门名称4]和[具体菌门名称5]。DT的α多样性指数高于LX。LX的优势核心属包括[具体属名称1]、[具体属名称2],DT的优势核心属包括[具体属名称3]、[具体属名称4]和[具体属名称5]。在LX中,病害抑制菌门([具体菌门名称1]、[具体菌门名称2]和[具体菌门名称3])和潜在的植物促生根际细菌(PGPR)的总相对丰度均显著高于DT。我们还确定了在[该中药材]根际中过度呈现的介导植物 - 微生物和微生物 - 微生物相互作用、营养获取以及植物生长促进的微生物功能特征。我们的研究结果为研究DT和LX种植的[该中药材]的有益微生物和病原体提供了有价值的参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ffa/8219928/0841cfd6165b/fmicb-12-673512-g001.jpg

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