School of the Environment and Safety Engineering, and Collaborative Innovation Center of Technology and Material of Water Treatment, Jiangsu University, Zhenjiang, 212013, PR China.
Fujian Key Laboratory of Agro-Products Quality & Safety, Fuzhou, 350003, PR China.
Mikrochim Acta. 2024 Oct 23;191(11):693. doi: 10.1007/s00604-024-06751-0.
By preparing Cu@CuO aerogel as a nanozyme which exhibits prominent peroxidase-like (POD) activity, an innovative homogeneous electrochemistry (HEC) coupled with the colorimetry dual-model sensing strategy is proposed to detect perfluorooctane sulfonate (PFOS) for the first time. Cu@CuO aerogel accelerates the oxidation process of colorless o-phenylenediamine to form yellow 2,3-diaminophenazinc (DAP), and meanwhile, DAP as an electroactive substance creates a reduction peak current upon the electrochemical measurements. Interestingly, in the presence of PFOS, the POD activity of Cu@CuO aerogel is inhibited since the specific coordination between PFOS and Cu(II) can cover the active sites, resulting in the color of the sensing system becoming light and the peak current of DAP decreasing. This innovative dual-mode detection method showed excellent electrochemical detection of PFOS in the concentration range 10.0 ~ 1125.0 nM with a limit of detection (LOD) as low as 3.3 nM and a LOD of 20.8 nM in the colorimetric detection in the range 62.3 ~ 875 nM. Furthermore, the sensor was successfully used for the analysis of real samples with an RSD value ≤ 6.5%. The successful application of this two-mode sensing method for the determination of PFOS holds promise for the detection of other contaminants in the future.
通过制备具有显著过氧化物酶样（POD）活性的 Cu@CuO 气凝胶作为纳米酶，首次提出了一种创新的均相电化学（HEC）结合比色双模式传感策略来检测全氟辛烷磺酸（PFOS）。Cu@CuO 气凝胶加速无色邻苯二胺的氧化过程，形成黄色 2,3-二氨基吩嗪（DAP），同时，DAP 作为电活性物质，在电化学测量时会产生还原峰电流。有趣的是，在 PFOS 存在的情况下，Cu@CuO 气凝胶的 POD 活性受到抑制，因为 PFOS 与 Cu(II) 之间的特异性配位可以覆盖活性位点，导致传感系统的颜色变浅，DAP 的峰电流减小。这种创新的双模式检测方法在 10.0 至 1125.0 nM 的浓度范围内对 PFOS 表现出优异的电化学检测性能，检测限（LOD）低至 3.3 nM，在 62.3 至 875 nM 的比色检测范围内 LOD 低至 20.8 nM。此外，该传感器还成功用于实际样品的分析，其相对标准偏差（RSD）值≤6.5%。该双模式传感方法成功应用于 PFOS 的测定，有望为未来检测其他污染物提供参考。
