Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Campus do Pici, CE, 60455-760, Fortaleza, Brasil.
Laboratório de Produtos Naturais, Centro de Ciência e Tecnologia, Universidade Estadual do Ceará, Campus Itaperi, 60714-903, Fortaleza, CE, Brasil.
Daru. 2023 Dec;31(2):183-192. doi: 10.1007/s40199-023-00473-z. Epub 2023 Aug 28.
Clove volatile oil (CVO) and its major compound, eugenol (EUG), have anxiolytic effects, but their clinical use has been impaired due to their low bioavailability. Thus, their encapsulation in nanosystems can be an alternative to overcome these limitations.
This work aims to prepare, characterize and study the anxiolytic potential of CVO loaded-nanoemulsions (CVO-NE) against anxious-like behavior in adult zebrafish (Danio rerio).
The CVO-NE was prepared using Agaricus blazei Murill polysaccharides as stabilizing agent. The drug-excipient interactions were performed, as well as colloidal characterization of CVO-NE and empty nanoemulsion (B-NE). The acute toxicity and potential anxiolytic activity of CVO, EUG, CVO-NE and B-NE against adult zebrafish models were determined.
CVO, EUG, CVO-NE and B-NE presented low acute toxicity, reduced the locomotor activity and anxious-like behavior of the zebrafish at 4 - 20 mg kg. CVO-NE reduced the anxious-like behavior of adult zebrafish without affecting their locomotor activity. In addition, it was demonstrated that anxiolytic activity of CVO, EUG and CVO-NE is linked to the involvement of GABAergic pathway.
Therefore, this study demonstrates the anxiolytic effect of CVO, in addition to providing a new nanoformulation for its administration.
丁香挥发油（CVO）及其主要化合物丁香酚（EUG）具有抗焦虑作用，但由于其生物利用度低，其临床应用受到限制。因此，将其包封在纳米系统中可以作为克服这些限制的一种替代方法。
本工作旨在制备、表征并研究负载丁香挥发油的纳米乳（CVO-NE）对成年斑马鱼（Danio rerio）焦虑样行为的抗焦虑潜力。
使用姬松茸多糖作为稳定剂制备 CVO-NE。对 CVO-NE 和空白纳米乳（B-NE）进行了药物-赋形剂相互作用以及胶体特性研究。测定了 CVO、EUG、CVO-NE 和 B-NE 对成年斑马鱼模型的急性毒性和潜在抗焦虑活性。
CVO、EUG、CVO-NE 和 B-NE 均表现出低急性毒性，可降低斑马鱼的运动活性和焦虑样行为，其作用浓度在 4-20mg/kg。CVO-NE 可降低成年斑马鱼的焦虑样行为，而不影响其运动活性。此外，研究还表明 CVO、EUG 和 CVO-NE 的抗焦虑活性与 GABA 能途径的参与有关。
因此，本研究证明了 CVO 的抗焦虑作用，并为其给药提供了一种新的纳米制剂。
