Guo Wei-Feng, Guo Dan-Dan, Li Fen, Shang Shen-Zhai, Li Ting-Wan, Tang Ying-Chao, Jiang Man, Xu Fu-Chun, Gao Wei
Agricultural College, Tarim University, Alaer, Xinjiang, People's Republic of China.
National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
Plant Cell Rep. 2023 Nov;42(11):1833-1836. doi: 10.1007/s00299-023-03061-2. Epub 2023 Aug 29.
The extensive application of CRISPR in cotton was limited due to the labor-intensive transformation process. Thus, we here established a convenient method of CRISPR in cotton by CLCrV-mediated sgRNA delivery.
由于转化过程需要大量人力,CRISPR在棉花中的广泛应用受到限制。因此,我们在此建立了一种通过CLCrV介导的sgRNA传递在棉花中应用CRISPR的简便方法。